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H Obata, T Oka, F Yoshitomi; Impression Cytology of Meibomian Gland Secretion . Invest. Ophthalmol. Vis. Sci. 2002;43(13):60.
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Purpose: We examined the cytologic feature of meibomian gland secretion, meibum in comparison with appearance. In order to avoid the loss of the sample during the preparation, impression cytology technique was used. Methods: The sampling was made on fifty elderly outpatients (mean age: 74.2 years, with a range from 61 to 94) after their informed consent. Meibum was gently pressed out by using Yoshitomi Meibomian Gland Compressor and collected by nitrocellulose membrane on lid margin. Papanicolaou staining was performed after fixation in isopropyl alcohol. The secretion was divided into three groups; oily, creamy, and toothpaste-like, and classified the color into yellow, yellow-white, and white. Results: There were 24 cases in oily group, 18 cases in cream-like group, and 8 cases in toothpaste-like group, and there was no age difference between each group. The keratinized materials were observed in almost all cases although there is a difference of quantity, and cellular components were not detected in most cases. There was no correlation between the color of meibum and the cytologic feature. Conclusion: It turned out that the keratinized materials were contained in many elderly people's meibum. It was thought that the difference in the color of meibum reflected the change of lipid composition of meibomian gland secretion.
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