December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Effect of Eye Wash Agent on Rabbit Tear Film Stability
Author Affiliations & Notes
  • S Nakamura
    Research Center OPHTECS Corporation Toyooka Japan
  • S Okada
    Research Center OPHTECS Corporation Toyooka Japan
  • F Saito
    Research Center OPHTECS Corporation Toyooka Japan
  • Footnotes
    Commercial Relationships   S. Nakamura, None; S. Okada, None; F. Saito, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 93. doi:
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      S Nakamura, S Okada, F Saito; Effect of Eye Wash Agent on Rabbit Tear Film Stability . Invest. Ophthalmol. Vis. Sci. 2002;43(13):93.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose:Eye lotion is commonly used to relief ocular fatigue by bathing the ocular surface. Treatment with an eye lotion withdraws all of the tear immediately, but there is less known about effect on tear film instability after treatment. To clarify this, we examined the effect of eye lotion and the preservatives, generally contained in commercial eye lotions on rabbit tear film stability. Methods:Seven Japanese white rabbits (7 eyes) were used in each group respectively. After bathing the rabbit eye for 30 seconds with 10 ml of two different types of commercial eye lotion, preservative-free artificial tear eye drop or 0.1% - 0.001% benzalconium chloride containing phosphate buffered solution under anesthesia. The appearance of tear break up area (TBA) following forced blinking was monitored using a specular reflection videorecording system (DR-1, Kowa, Japan). TBA (mm2/mm2) was calculated by analyzing images of the tear film. Results:The appearance of TBA treatment with preservative free artificial tear eye drop as moderate as non-treated eyes. In contrast, both commercial eye lotions increased TBA drastically. The area of TBA 30 seconds after the forced blinking following application of commercial eye lotions (0.122±0.027, 0.092 ± 0.012) was significant (p< 0.05), compared to preservate free artificial tear eye drop (0.023 ± 0.005) or non-treated eyes (0.003 ± 0.005). Benzalconium chloride increased TBA in dose-dependent manner. Conclusion:Our results suggest that tear film becomes extensively unstable after bathing with eye lotions, and preservative seem to be closely associate with tear film disordes. To prevent this, treatment with preservative-free artificial tear eye drop will be available.

Keywords: 376 cornea: tears/tear film/dry eye • 372 cornea: epithelium • 375 cornea: surface mucins 
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