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P Hamard, C Debbasch, F Brignole, C Baudouin; Toxicity of Preserved and Unpreserved Antiglaucoma Eyedrops on Human Cultured Trabecular Cells . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1035.
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Purpose: To compare the toxicity of short-time application of antiglaucoma eyedrops with and without benzalkonium chloride preservative (respectively BAC+ and BAC-) in an in vitro model of human trabecular cells. Material and Methods: Human immortalized trabecular cells lines (Abbot Clark, Alcon laboratories, Fort Worth, TX) were treated for 15 min. with 0.5% timolol BAC+ and BAC-, 0.25% betaxolol suspension BAC+ and BAC-, 0.005% latanoprost, and 0.01% BAC in 1:10 and 1:100 dilutions. Cell size and the expression of an apoptotic marker Apo 2.7 were evaluated 24 hours after by flow cytometry. Results: All tested drugs in a 1:10 and 1:100 dilutions, except 0.5% timolol BAC-, induced significant trabecular cell size decrease as compared to controls (p<0.05), more pronounced with 0.001% BAC (p<10-4). Preserved eye drops induced significant cell size decrease as compared to nonpreserved eyedrops in a 1:10 dilution (p<0.04). When increasing drug concentration from 1:100 to 1:10, trabecular cell size significantly decreased only with preservated eyedrops (p<0.05). Apo 2.7 expression was significantly increased with 0.001% BAC (p<10-4) and 0.0001% BAC (p<0.005). 0.005% latanoprost in a 1:10 dilution induced significant Apo 2.7 overexpression as compared to 0.5% timolol BAC- and 0.25% betaxolol BAC- in a 1:10 dilution (p<0.05). Conclusion: Benzalkonium chloride induces in vitro cytotoxic damage to trabecular cells with some characteristics of apoptosis.
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