December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
Expression of Galectin-1 and Galectin-3 in the Human Trabecular Meshwork
Author Affiliations & Notes
  • MP Fautsch
    Ophthalmology Mayo Clinic Rochester MN
  • AO Silva
    Ophthalmology Mayo Clinic Rochester MN
  • DH Johnson
    Ophthalmology Mayo Clinic Rochester MN
  • Footnotes
    Commercial Relationships   M.P. Fautsch, None; A.O. Silva, None; D.H. Johnson, None. Grant Identification: Support: NIH grant EY07065 and RPB, Inc.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 1057. doi:
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      MP Fautsch, AO Silva, DH Johnson; Expression of Galectin-1 and Galectin-3 in the Human Trabecular Meshwork . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1057.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose:Galectins are a family of carbohydrate binding proteins involved in a variety of biological processes including cell-cell and cell-matrix interactions. We examined galectin-1 and galectin-3 to determine if galectins are expressed in the human trabecular meshwork. Methods:Galectin-1 and galectin-3 expression was examined by RT-PCR and Western blot analysis in human trabecular meshwork. Cultured human trabecular meshwork cells treated with and without dexamethasome were analyzed by Northern blot analysis. Immunohistochemistry of galectin-1 and galectin-3 was analyzed in normal and glaucomatous tissue. Myocilin was used as a comparative control. Results:Galectin-1 and galectin-3 were found throughout the trabecular meshwork in both normal and glaucomatous eyes. In addition, both galectin-1 and galectin-3 were found in the cells lining Schlemm's canal. In cultured trabecular meshwork cells, galectin-1 and galectin-3 are slightly downregulated by dexamethasome. In contrast, myocilin expression was increased in dexamethasome treated cells. Conclusion:Galectin-1 and galectin-3 are present in human trabecular meshwork. These proteins may be involved in cellular or extracellular matrix organization through their potential interactions with glycoconjugates such as glycoproteins, glycolipids, and proteoglycans.

Keywords: 601 trabecular meshwork • 476 molecular biology • 417 gene/expression 

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