December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Rescue Of Photorecetors From Retinal Detachment-induced Damage By Adeno-associated Virus Vector Expressing Glial Cell Line-derived Neurotrophic Factor
W-C Wu; C-C Lai; S-L Chen; X Xiao; T-L Chen; RJ F Tsai; S-W Kuo; Y-P Tsao
Author Affiliations & Notes
  • W-C Wu
    Department of Ophthalmology Chang-Gung Memorial Hospital Taoyuan Taiwan Republic of China
  • C-C Lai
    Department of Ophthalmology Chang-Gung Memorial Hospital Taoyuan Taiwan Republic of China
  • S-L Chen
    Department of Microbiololgy and Immunology National Defense Medical Center Taipei Taiwan Republic of China
  • X Xiao
    Department of Molecular Genetics and Biochemistry University of Pittsburgh Pittsburgh PA
  • T-L Chen
    Department of Ophthalmology Chang-Gung Memorial Hospital Taoyuan Taiwan Republic of China
  • RJ F Tsai
    Department of Ophthalmology Chang-Gung Memorial Hospital Taoyuan Taiwan Republic of China
  • S-W Kuo
    Department of Medical Research Veteran\#8217;s General Hospital Taipei Taiwan Republic of China
  • Y-P Tsao
    Department of Ophthalmology Chang-Gung Memorial Hospital Taoyuan Taiwan Republic of China
  • Footnotes
    Commercial Relationships   W. Wu, None; C. Lai, None; S. Chen, None; X. Xiao, None; T. Chen, None; R.J.F. Tsai, None; S. Kuo, None; Y. Tsao, None. Grant Identification: Chang Gung Memorial Hospital Medical Research Grant CMRP-347
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 650. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      Rescue Of Photorecetors From Retinal Detachment-induced Damage By Adeno-associated Virus Vector Expressing Glial Cell Line-derived Neurotrophic Factor
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      W-C Wu, C-C Lai, S-L Chen, X Xiao, T-L Chen, RJ F Tsai, S-W Kuo, Y-P Tsao; Rescue Of Photorecetors From Retinal Detachment-induced Damage By Adeno-associated Virus Vector Expressing Glial Cell Line-derived Neurotrophic Factor . Invest. Ophthalmol. Vis. Sci. 2002;43(13):650.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Abstract: : Purpose: To examine the protective effect of glial cell line-derived neurotrophic factor (GDNF) on retinal detachment (RD) induced photoreceptor damage by ways of gene delivery. Methods: Gene delivery to photoreceptors was achieved by subretinal injection of recombinant AAV expressing GDNF (rAAV-GDNF) in the right eyes and AAV expressing E. coli lacZ (rAAV-lacZ) in the left eyes of Lewis rats. RD in bilateral eyes was induced with subretinal injection of high-density vitreous substitute in the temporal retina 3 weeks after gene delivery. The synthesis and accumulation of GDNF within retina was monitored 3 weeks after RD by immunohistochemistry and enzyme-linked immunosorbent assay (ELISA)espectively. The rescue of photoreceptors was evaluated by monitoring the preservation of the thickness of photoreceptor outer segment (OS) and outer nuclear layer (ONL). Apoptosis of photoreceptors was studied using the TdT-dUTP terminal nick-end labeling (TUNEL) method 2 days after RD. Müller cell activity was checked using the immunohistochemistry with glial fibrillary acidic protein (GFAP) antibody. Results: The gene delivery was demonstrated by immunohistochemistry study. High levels of neurotrophic factors were produced in retina by ELISA study. Photoreceptor OS degeneration and the gradual shortening of the ONL were noted after RD in all the eyes. However, GDNF-treated eyes retained longer OS than controlled eyes 7 days (p=0.012) and 28 days (p=0.008) after RD. ONL was also longer in GDNF-treated eyes than in controlled eyes 7 days (p=0.012) and 28 days (p=0.008) after RD. GDNF-treated eyes had statistically less apoptotic cells than control eyes in photoreceptor layer (p=0.043). Müller cell activity was less prominent in GDNF-treated group, indicating less scar-formation activity. Conclusion: GDNF is a potential factor that can protect photoreceptors from degeneration. GDNF gene therapy may be a valuable adjuvant to current treatments in certain complicated forms of retinal detachment.

Keywords: 563 retinal detachment • 517 photoreceptors • 489 neuroprotection 
© 2002, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept