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XD Sun, X Zhang; Neuorprotection by NGF in Experimental Retinal Detachment . Invest. Ophthalmol. Vis. Sci. 2002;43(13):654.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To study the protective effect and mechanism of nerve growth factor (NGF) on retina in experimental retinal detachment (RD). Methods: Eighty two Sprague-Dawely rats were used as RD animal model by infused 0.1% sodium hyaluronate under the neurosensory retina. Those rats were divided into NGF group and control group. NGF 5µg/5µl/eye and PBS 5µl/eye as control were respectively injected into vitreous every 4 days after RD. On postoperative time (hours and days) 1.5h, 3h, 6h, 12h, 1d, 2d, 4d, 8d, 16d, 32d, SD rats were sacrificed and eyes were extracted. The effect of NGF on retina was assessed by electron, light microscopy and cell counter. Expressions of NGF and its receptor TrkA, intermediate filament proteins were identified by immunohistochemistry. SPSS 10.0 was used as statistical analysis. Results: Expressions of NGF and TrkA were clearly identified around outer nuclear layer in normal control and RD groups and increased after RD. Histomorphology and cell counter studies showed no significant difference between two groups in early days after RD, but in 16d and 32d groups which showed significantly lower lesion in NGF groups than in control groups, especially in outer and inner segments of photoreceptor cells. NGF groups also showed lower labeling intensity of glial fibrillary acidic protein and vimentin in cytoplasm and clear structure of M黮ler cells (p<0.05). Conclusion: Normal rat retina express NGF and its receptor TrkA. Intravitreal injection exogenous NGF protect retinal cells from degeneration in experimental RD. Exogenous NGF injection enhance inner protection mechanism from NGF after RD.
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