December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Nitric Oxide Synthase-2 in Muller Glial Cells Induced by Elevated Pressure In Vitro
Author Affiliations & Notes
  • Y Suzuki
    Department of Ophthalmology University of Tokyo Graduated School of Medicine Bunkyo-ku Japan
  • H Yamada
    Department of Ophthalmology University of Tokyo Graduated School of Medicine Bunkyo-ku Japan
  • R Inoue
    Department of Ophthalmology University of Tokyo Graduated School of Medicine Bunkyo-ku Japan
  • M Ohashi
    Department of Ophthalmology University of Tokyo Graduated School of Medicine Bunkyo-ku Japan
  • K Kashiwagi
    Department of Ophthalmology Yamanashi Medical University Yamanashi Japan
  • Footnotes
    Commercial Relationships   Y. Suzuki, None; H. Yamada, None; R. Inoue, None; M. Ohashi, None; K. Kashiwagi, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 659. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Y Suzuki, H Yamada, R Inoue, M Ohashi, K Kashiwagi; Nitric Oxide Synthase-2 in Muller Glial Cells Induced by Elevated Pressure In Vitro . Invest. Ophthalmol. Vis. Sci. 2002;43(13):659.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: Recently, nitric oxide (NO) has received much attention as an important mediator with pathologic roles in glaucoma. Excessive NO, particularly synthesized by NOS-2 in retinal Muller cells, may be neurodestructive in patients with glaucoma. In this report, we studied NOS-2 mRNA induction and NO production in Muller cells in response to elevated pressure. Methods: Muller cells were obtained from enucleated rat eyes at postnatal day 6 to 8. Cell were seeded into dishes, and maintained at 37ºC in humidified atmosphere containing 5% CO2 and 95% air. To investigate the effect of elevated pressure in the gene transcription of NOS-2, Muller Cells were placed in sealed chamber at elevated pressure (+152mmHg)for 2 hours. The induction of NOS-2 was evaluated by RT-PCR. NO production was also evaluated with DAF (diaminofluorescein-2). Results: Immediately after exposing to elevated pressure, mRNA level of NOS-2 did not change compared with control. When maintained at the atmospheric pressure, mRNA level of NOS-2 showed approximately 4-fold increased at 2 hours. At 4 hours, it decreased to the control level. NO production monitored by DAF also showed similar time course. Conclusion: NOS-2 in Muller cells was induced by elevated pressure in vitro. The excessive NO produced by NOS-2 in Muller cell is neurodestructive and may contribute to retinal ganglion cell damage.

Keywords: 491 nitric oxide • 479 Muller cells • 489 neuroprotection 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×