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H Mochizuki, I Sakamoto, T Kato, Y Tsumamoto, H Yamashita, HK Mishima; Induction of Nitric Oxide in Retinal Müller Glial Cells by -crystallin . Invest. Ophthalmol. Vis. Sci. 2002;43(13):660.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To determine whether stress-inducible small heat shock protein, α-crystallin, induces in vitro activation of retinal Müller glial (RMG) cells cultured from newborn rat retina. Methods: Müller cells were isolated from neonatal Wister rat retina and cultured as previously described (Hicks and Courtois, 1990). Cells were exposed to increasing concentrations of lipopolysaccharide, interferon-γ and α-crystallin. At selected intervals after exposure, nitrite levels in media were evaluated by the Griess reaction. Results: Exposure of RMG cells to α-crystallin resulted in an increased production of nitric oxide (NO). Conclusions: The results demonstrate in vitro activation of RMG cells by α-crystallin induces NO release. α-crystallin could be implicated in some retinal diseases.
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