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N Ogata, T Otsuji, M Wada, N Jo, M Matsumura; Expression Of Pigment Epithelium Derived Factor In Normal Adult Rat Eye And Experimenral Choroidal Neovascularization . Invest. Ophthalmol. Vis. Sci. 2002;43(13):687.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:Pigment epithelium-derived factor (PEDF) is a protein produced by the retinal pigment epithelial (RPE) cells. Recent studies have implicated PEDF in activities that are inhibitory to angiogenesis. In this study, we investigated the expression of PEDF in normal rat eyes and in eyes with an experimentally-induced choroidal neovascularization and compared it to the expression of vascular endothelial growth factor (VEGF). Methods:Choroidal neovascularization was induced by laser photocoagulation in rat eyes. At intervals of up to 2 weeks after photocoagulation, the eyes were removed and prepared for in situ hybridization and immunohistochemical study. In situ hybridization was performed with digoxigenin-labeled PEDF riboprobes. Protein expression of PEDF and VEGF was studied immunohistochemically. Results:In normal adult rat eyes, PEDF mRNA was observed mainly in the corneal epithelial and endothelial cells, lens epithelial cells, ciliary epithelial cells, retinal ganglion cells, and the RPE cells. During the development of choroidal neovascularization, PEDF and also VEGF protein were strongly detected in the laser lesions at 3 days after photocoagulation then gradually declined. However, PEDF was expressed in the RPE cells that proliferated and covered the neovascular tissues at 2 weeks while VEGF protein was weakly expressed in endothelial cells in the choroidal neovascularizarion. Conclusion:PEDF is expressed in different cell types of normal rat eyes. The expression of PEDF was detected in the choroidal neovascular tissues induced by photocoagulation, and these findings suggest that PEDF may modulate the process of choroidal neovascularization.
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