December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Migration And Proliferation Of Human Retinal Pigment Epithelial Cells Induced By Alteration Of Protein Kinase C Activity On Different Extracellular Matrices
Author Affiliations & Notes
  • I Nachbar
    Department of Ophthalmology
    Dr Horst-Schmidt-Kliniken WFK Wiesbaden Germany
  • F Sistani
    Dept of Opthalmology
    Dr Horst-Schmidt-Kliniken WFK Wiesbaden Germany
  • UH Steinhorst
    Dept of Opthalmology DrHorst-Schmidt-Kliniken WFK Wiesbaden Germany
  • Footnotes
    Commercial Relationships   I. Nachbar, None; F. Sistani, None; U.H. Steinhorst, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 691. doi:
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      I Nachbar, F Sistani, UH Steinhorst; Migration And Proliferation Of Human Retinal Pigment Epithelial Cells Induced By Alteration Of Protein Kinase C Activity On Different Extracellular Matrices . Invest. Ophthalmol. Vis. Sci. 2002;43(13):691.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Rentinal pigment epithelium-loss is a part of the pathological process in age-related macular degeneration (ARMD). A potential therapeutic option might be to stimulate migration of human retinal pigment epithelial cells (hRPE) from the periphery to the damaged macular area. We investigated the migration of hRPE cells induced by alteration of protein kinase C activity (PKC) using various extracellular matrices (ECM). Methods: The effect of phorbol 12-mystriate 13-acetate (PMA) as an PKC activator and staurosporine as an PKC inhibitor on hRPE cell migration was investigated in vitro on different ECMs (glass, fibronectin and collagen type IV). After 24h, cell migration was measured by counting those cells that had entered a previously denuded area of the slide. All cultures were exposed to proliferating cell nuclear antigen (PCNA) using the APAAP-technique, permitting to identify proliferation activity. Results: Staurosporine reduced the number of hRPE cells entering the denuded area significantly (p< 0,05) compared with controls. In contrast PMA increased the number of migrating hRPE cells on collagen type IV. However, no such effect was found on glass or fibronectin. Conclusion: Activated PKC increases migratory activity of hRPE cells in vitro. This effect depends of the type of ECM on which the cells are grown. However, in our experimental settings proliferation always played an essential role in wound healing which was independent of the PKC activity or ECM. None of the substances used, stimulated migration of hRPE cells without simultaneously increasing cell proliferation.

Keywords: 567 retinal pigment epithelium • 631 wound healing • 308 age-related macular degeneration 
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