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SC Park, H Kim, Y Kim, J Suh; Roles of Stress-induced c-met/HGF Receptor in ARPE-19 Cells . Invest. Ophthalmol. Vis. Sci. 2002;43(13):720.
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Purpose: The hepatocyte growth factor (HGF/SF) receptor, c-met, regulates motility, mitogenesis, and morphogenesis in a cell type dependent fashion. We report the role of stress-induced HGF receptor in the human retinal pigment epithelial cell (hRPE) line ARPE-19. Methods: RPE cells cultured under common or serum-free conditions. Southern and Western blot analyses were performed to determine the expression patterns of HGF/SF and its receptor in cultured hRPE and serum-starved ARPE-19 cells. The proliferation pattern by c-met in serum-starved ARPE-19 was analyzed using neutralizing monoclonal antibody (2ug/ml). Results: In primary cultured human RPE cells, c-met was expressed progressively from early culture passage. HGF was expressed only at late culture passage (p10). In ARPE-19, c-met was expressed only in serum-starved condition. Neutralizing antibody to c-met inhibited the proliferation of serum-deprived ARPE-19 by 64.5% (n=9, S.D. 5.5%). Conclusion: We suggest that c-met induced under non-physiologic conditions seems to have significant effect on RPE cell proliferation.
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