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S-M Choi, J-S Choi, M-O Park, B-J Gwag, C-K Joo; Protection of Ischemia-Reperfusion Induced Cell Death by Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) in the Rat Retina . Invest. Ophthalmol. Vis. Sci. 2002;43(13):770.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: The degeneration of retinal neurons results in loss of vision. It has been known that Non Steroidal Anti-Inflammatory Drugs (NSAIDs) can protect the neuron from ischemic damage. The purpose of this study was to investigate the protective effect of NSAIDs in the rat retinal ischemia. Methods: Retinal ischemia in Sprague Dawley rats was induced by high intraocular pressure at 160 mmHg for 60 minutes after intra-ocular injection of saline, aspirin (5 to 20 mM), sulfasalazine (1 to 5 mM) or sulindac (0.01 to 0.1 mM). For morphological study, the retinas were embedded in epon 24 hours after ischemic injury. To determine neuronal survivorship in the retinal layers, the number of viable neurons was counted in 100µm X 25µm square and examined. Results: The intravitreal injections of aspirin, sulfasalazine or sulindac attenuated the ischemic neuronal degeneration in dose dependent. The protective effect of aspirin at concentration of 20 mM was observed to be 73%5.4 and 80%2.5 in ganglion cell layer (GCL) and inner nuclear cell layer (INL), respectively. Treatment with 5 mM of sulfasalazine showed the protective effect of 53%8.8 in GCL and 74%5.3 in INL whereas 65%9.4 in GCL and 84%3.0 in INL were observed for 0.1 mM of sulindac treated group. Conclusion: These data suggest that NSAIDs (aspirin, sulfasalazine or sulindac) can be the potential drugs to protect the retina neurons injured by ischemia.
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