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G Wang, JZ Li, FS Zhang, T Li, QJ Zhang, XS Xiao, SQ Li, XY Jia, L Guo, XM Guo; Mutations of the Zinc Finger Protein 161 Gene in Chinese With or Without High Myopia . Invest. Ophthalmol. Vis. Sci. 2002;43(13):823.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: Zinc finger protein has been shown to related with experimental myopia. A zinc finger protein (ZFP161, omim602126) is located on 18p11.31 where a locus for high myopia (MYP2) has been determined but causative gene has not yet been identified. This study was to search for genes responsible for MYP2 and to analyze the sequence variation of ZFP161 in probands with high myopia. Methods: Genomic DNA was prepared from probands of 211 family with high myopia (<=-6.0 diopters ) and 116 unrelated persons without high myopia. The coding regions of ZFP161 were analyzed by using PCR- heteroduplex -SSCP analysis and sequencing. Results: Variations at codon56GCC→GCA (Ala56Ala) and IVS1-58∼59 ATins were detected. The former one is a nonsense heterozygous mutation identified in 5 of the 211 probands with high myopia and 3 of the 116 individuals without high myopia. The other is a heterozygous AT insertion in the intron but none of the 116 controls. Family analysis showed this mutation does not present in her parents who have no high myopia. Conclusion: No evidence shows that the ZFP161 gene is responsible for high myopia.
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