December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
Glut8 Expression in the Blood Retinal Barrier
Author Affiliations & Notes
  • DN Henry
    Physiology and Pediatrics/Human Development
    Michigan State University East Lansing MI
  • JV Busik
    Physiology Michigan State Universtiy East Lansing MI
  • D Botolin
    Michigan State University East Lansing MI
  • MB Grant
    Pharmacology and Therapeutics University of Florida Gainesville FL
  • N Gorovits
    Biochemistry Albert Einstein School of Medicine New York NY
  • M Charron
    Biochemistry Albert Einstein School of Medicine New York NY
  • Footnotes
    Commercial Relationships   D.N. Henry, None; J.V. Busik, None; D. Botolin, None; M.B. Grant, None; N. Gorovits, None; M. Charron, None. Grant Identification: Neuroscience Program Gant-Michigan State University
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 904. doi:
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    • Get Citation

      DN Henry, JV Busik, D Botolin, MB Grant, N Gorovits, M Charron; Glut8 Expression in the Blood Retinal Barrier . Invest. Ophthalmol. Vis. Sci. 2002;43(13):904.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose:Facilitative glucose transporters (GLUTs) of the blood-retinal barrier (BRB) are important in the function and pathology of the retina. GLUT8 is a newly discovered facilitative glucose transporter in human and animals. GLUT8 is modulated in brain-ganglion cells in diabetic rats but has never been reported in the BRB where GLUT1 is believed to be the exclusive GLUT. Methods:GLUT8 expression was determined by confocal microscopy, Northern, western, plasma membrane and cytosolic fractions and by in situ analysis of BRB. Results:GLUT8 (like GLUT1) was present in the inner and outer BRB and the ganglion layer in retina. Approximately 25% of GLUT8 is present in the plasma membrane of the BRB and the remainder in microsomal (perinuclear Golgi) fractions. Conclusion:GLUT8 is expressed at high levels in the BRB and distributed in both the plasma membrane and Golgi. GLUT1 is not the exclusive GLUT of the BRB. Possible regulation of expression of GLUT8 by glucose, cytokines and growth factors may contribute to the disruption of BRB in diabetes.

Keywords: 342 cell membrane/membrane specializations • 331 blood supply • 388 diabetic retinopathy 

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