December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Optical Imaging Demonstrates Amelioration of Cortical Visual Function in Dystrophic Rats Following Transplantation of Human Immortalised RPE Cells
Author Affiliations & Notes
  • C Gias
    VTRG Psychology
    University of Sheffield Sheffield United Kingdom
  • M Jones
    VTRG Psychology
    University of Sheffield Sheffield United Kingdom
  • D Keegan
    Institute of Ophthalmology UCL London United Kingdom
  • P Adamson
    Institute of Ophthalmology UCL London United Kingdom
  • J Greenwood
    Institute of Ophthalmology UCL London United Kingdom
  • RD Lund
    Moran Eye Center University of Utah Salt Lake City UT
  • J Martindale
    Neuroscience Imaging Group
    University of Sheffield Sheffield United Kingdom
  • D Johnston
    Neuroscience Imaging Group
    University of Sheffield Sheffield United Kingdom
  • J Mayhew
    Neuroscience Imaging Group
    University of Sheffield Sheffield United Kingdom
  • P Coffey
    VTRG Psychology
    University of Sheffield Sheffield United Kingdom
  • Footnotes
    Commercial Relationships   C. Gias, None; M. Jones, None; D. Keegan, None; P. Adamson, None; J. Greenwood, None; R.D. Lund, None; J. Martindale, None; D. Johnston, None; J. Mayhew, None; P. Coffey, None. Grant Identification: Wellcome Trust
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 921. doi:
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      C Gias, M Jones, D Keegan, P Adamson, J Greenwood, RD Lund, J Martindale, D Johnston, J Mayhew, P Coffey; Optical Imaging Demonstrates Amelioration of Cortical Visual Function in Dystrophic Rats Following Transplantation of Human Immortalised RPE Cells . Invest. Ophthalmol. Vis. Sci. 2002;43(13):921.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: The aim of this series of experiments was to use optical imaging technology to investigate visual cortical function in dystrophic Royal College of Surgeons (RCS) rats following sub-retinal transplantation of human immortalised RPE cells. Methods: 3-4 week old dystrophic RCS rats received sub-retinal injections of a human immortalised RPE cell line (h1RPE7). At 5 months of age animals were anaesthetised and the skull overlying the right visual cortex was thinned to translucency. A 12 bit CCD camera was used to image the changes in remitted illumination from visual cortex. A number of visual stimuli were presented to the rats left eye: flickering full screen flash plus horizontal and vertical drifting gratings of 0.05, 0.15 and 0.4 cycles/degree. Data were averaged over 30 trials. Following location of activity, spectroscopy was performed to further examine the hemodynamic response to stimulation. Furthermore, visually evoked potentials were recorded from both surface and layer IV of the visual cortex. Results: In transplanted animals responses could be detected to full screen flash and various gratings. Single wavelength imaging detected cortical darkening following stimulus presentation, which indicated an increase in blood volume characteristic of neural activity. The presence of hemodynamic responses was confirmed by imaging spectroscopy. Surface and layer IV visually evoked potentials were also recorded from the 'optically active' region. Control dystrophic animals did not show this pattern of visual cortical function. Conclusion: These data confirm the efficacy of human immortalised RPE transplants and suggest optical imaging may be a useful tool for visual cortical functional assessment.

Keywords: 607 transplantation • 431 imaging/image analysis: non-clinical • 621 visual cortex 
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