December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
Photomelded Bruch's Membrane Patch Grafts: A Novel Concept To Treat Bruch's Membrane Defects In Age-related Macular Degeneration
Author Affiliations & Notes
  • TH Tezel
    Department of Ophthalmology and Visual Sciences Washington University Saint Louis MO
  • LV Del Priore
    Department of Ophthalmology Columbia University New York NY
  • HJ Kaplan
    Department of Ophthalmology & Visual Sciences University of Louisville Louisville KY
  • Footnotes
    Commercial Relationships   T.H. Tezel, None; L.V. Del Priore, None; H.J. Kaplan, None. Grant Identification: Unrestricted funds from RPB, Inc, N.Y., Commonwealth of Kentucky Research Challenge Trust Fund
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 925. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      TH Tezel, LV Del Priore, HJ Kaplan; Photomelded Bruch's Membrane Patch Grafts: A Novel Concept To Treat Bruch's Membrane Defects In Age-related Macular Degeneration . Invest. Ophthalmol. Vis. Sci. 2002;43(13):925.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Abstract: : Purpose:To determine the feasibility and optimum conditions to repair defects in the inner aspects of Bruch's membrane with photomelded Bruch's membrane patch grafts by using Rose Bengal dye in vitro. Methods:Six-millimeter explants of human peripheral inner collagenous layer were prepared from 10 aged (≷60 years-old) cadaver eyes. Exposed inner collagenous layers were coated with different concentrations of Rose Bengal (0.1-20 mM). Explants were then photomelded to each other by photoexciting Rose Bengal with a white cold light source using light intensity matched to vitrectomy endoilluminator for 3 minutes to avoid any retinal phototoxicity. Attachment strength was measured in a buoyancy chamber. Attached grafts were kept under 0.21 mN (milliNewtons) of constant tractional force for 3 days to test the stability of the melding process. Results:Three minutes of exposure to 35 mW of light at a distance of 40 millimeters resulted in photomelding of exposed human inner collagenous layers to each other at concentrations above 0.1 mM. Attachment strength increased up to 14.1 N/m2 at 1.5 and 5 mM concentrations of Rose Bengal. At higher concentrations the attachment strength decreased (5.7 N/m2 and 2.83 N/m2 at 10 and 20 mM, respectively) due to collagen denaturation. The minimum concentration of Rose Bengal dye to attain strong photomelding of human inner collagenous layer grafts to each other was 1.5 mM. Photomelded grafts remained attached during the 72 hours of observation at 37ºC despite continuous traction. Conclusion:Photomelding of Bruch's membrane patch grafts may open a new horizon in repairing the defects of aged and structurally damaged subfoveal BM. Incorporating Bruch's membrane patch grafts with RPE and/or stem cells may restore the RPE beneath the fovea and recover central vision in age-related macular degeneration.

Keywords: 308 age-related macular degeneration • 333 Bruch's membrane • 607 transplantation 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.