December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Corneal Response to Pseudomonas Aeruginosa Infection in the Absence of IL-10
NA Cole; M Krockenberger; F Stapleton; AJ Husband; MD P Willcox
Author Affiliations & Notes
  • NA Cole
    Cooperative Research Centre for Eye Research and Technology University of New South Wales Sydney Australia
  • M Krockenberger
    Veterinary Anatomy and Pathology University of Sydney Sydney Australia
  • F Stapleton
    Cooperative Research Centre for Eye Research and Technology University of New South Wales Sydney Australia
  • AJ Husband
    Veterinary Anatomy and Pathology University of Sydney Sydney Australia
  • MD P Willcox
    Cooperative Research Centre for Eye Research and Technology University of New South Wales Sydney Australia
  • Footnotes
    Commercial Relationships   N.A. Cole, None; M. Krockenberger, None; F. Stapleton, None; A.J. Husband, None; M.D.P. Willcox, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 942. doi:
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      NA Cole, M Krockenberger, F Stapleton, AJ Husband, MD P Willcox; Corneal Response to Pseudomonas Aeruginosa Infection in the Absence of IL-10 . Invest. Ophthalmol. Vis. Sci. 2002;43(13):942.

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Abstract

Abstract: : Purpose: To explore the role of IL-10 in the cornea during Pseudomonas aeruginosa corneal infection by examining the effects of the absence of IL-10 on the pathogenesis of this disease. Method: IL-10 gene knockout and wildtype mice were challenged by topical application of P. aeruginosa to wounded corneas. Corneas were examined clinically, microbiologically and histologically over 7 days. Additionally, cytokine and myeloperoxidase levels in corneas were determined. Results: The IL-10 gene knockout mice showed less severe corneal disease than the wild type mice, however, they had more extensive vascularisation of the cornea at 7 days. IL-10 gko mice had approximately five-fold reduction of the bacterial load at 7 days post-challenge compared to the wild type mice, with corresponding changes in chemokine, cytokine and VEG-F levels. Conclusion: The absence of IL-10, a pleiotropic cytokine with anti-inflammatory effects, leads to more effective control of bacterial infection of the cornea, but more extensive vascularisation. Local administration of anti-IL-10 antibody early in the infectious process may improve outcomes during this destructive disease of the cornea.

Keywords: 316 animal model • 380 cytokines/chemokines • 531 Pseudomonas 
© 2002, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2015 Association for Research in Vision and Ophthalmology.
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