December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Suppression of Preretinal Neovascularization by Recombinant Adeno-associated Virus Expressing Angiostatin
C-C Lai; W-C Wu; S-L Chen; X Xiao; T-C Tsai; S-J Huan; T-L Chen; R-F Tsai; Y-P Tsao
Author Affiliations & Notes
  • C-C Lai
    Department of Ophthalmology Chang Gung Memorial Hospital Kwei-Shan Taiwan Republic of China
  • W-C Wu
    Department of Ophthalmology Chang Gung Memorial Hospital Kwei-Shan Taiwan Republic of China
  • S-L Chen
    Department of Microbiology and Immunology National Defense Medical Center Taipei Taiwan Republic of China
  • X Xiao
    Department of Molecular Genetics and Biochemistry University of Pittsburgh Pittsburgh PA
  • T-C Tsai
    Department of Medical research Veteran\#8217;s General Hospita Taipei Taiwan Republic of China
  • S-J Huan
    Department of Microbiology and Immunology National Defense Medical Center Taipei Taiwan Republic of China
  • T-L Chen
    Department of Ophthalmology Chang Gung Memorial Hospital Kwei-Shan Taiwan Republic of China
  • R-F Tsai
    Department of Ophthalmology Chang Gung Memorial Hospital Kwei-Shan Taiwan Republic of China
  • Y-P Tsao
    Department of Ophthalmology Chang Gung Memorial Hospital Kwei-Shan Taiwan Republic of China
  • Footnotes
    Commercial Relationships   C. Lai, None; W. Wu, None; S. Chen, None; X. Xiao, None; T. Tsai, None; S. Huan, None; T. Chen, None; R. Tsai, None; Y. Tsao, None. Grant Identification: CMRP1106
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 1269. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      Suppression of Preretinal Neovascularization by Recombinant Adeno-associated Virus Expressing Angiostatin
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      C-C Lai, W-C Wu, S-L Chen, X Xiao, T-C Tsai, S-J Huan, T-L Chen, R-F Tsai, Y-P Tsao; Suppression of Preretinal Neovascularization by Recombinant Adeno-associated Virus Expressing Angiostatin . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1269.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Abstract: : Purpose: To test the efficacy of recombinant adeno-associated virus (rAAV) vector expressing mouse angiostatin in suppressing experimental preretinal neovascularization in an albino rat model. Methods: rAAV-angiostatin and control virus rAAV-lacZ were delivered in vivo by intravitreous injection on Sprague-Dawley rats and the delivery was confirmed by reverse-transcriptase polymerase chain reaction (RT-PCR). For suppression of preretinal neovascularization, preretinal neovascularization was created by retinal vein occlusion 21 days after the injection. The retinal vein occlusion was induced by photodynamic therapy with systemic injection of rose bengal (40mg/kg) followed by an argon green laser. The animals were sacrificed 10 days after the retinal vein thrombosis and perfused with FITC-labelled dextran to serve as neovascularization analysis. After perfusion, those samples with evidence of neovascularization were quantified using an image analyzer. The retinas were also embedded in paraffin and serially sectioned at 6µm. The samples were stained with hematoxylin and eosin. Nuclei above the internal limiting membrane were then count in a masked manner. Results: The sizes of preretinal neovascularization in rAAV-angiostatin injected eyes were significantly smaller than the controlled eyes. Moreover, the number of vitreous cells was also less in experimental eyes. Conclusion: rAAV-angiostatin can successfully suppress the experimental preretinal neovascularization in albino rats. The results showed the feasibility of gene therapy approach as treatment of preretinal neovascularization.

Keywords: 419 gene transfer/gene therapy • 566 retinal neovascularization • 448 ischemia 
© 2002, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept