December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Reduced Choroidal Neovascularization in Matrix Metalloproteinase-2 (MMP-2) Deficient Mice
Author Affiliations & Notes
  • LC Berglin
    Retina StEriks Eye Hospital Karolinska Institutet Stockholm Sweden
  • S Sarman
    Retina StEriks Eye Hospital Karolinska Institutet Stockholm Sweden
  • S Seregard
    Retina StEriks Eye Hospital Karolinska Institutet Stockholm Sweden
  • I van der Ploeg
    Research St Eriks Eye Hospital Karolinska Institutet Stockholm Sweden
  • A Kvanta
    Retina StEriks Eye Hospital Karolinska Institutet Stockholm Sweden
  • Footnotes
    Commercial Relationships   L.C. Berglin, None; S. Sarman, None; S. Seregard, None; I. van der Ploeg, None; A. Kvanta, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 1290. doi:
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      LC Berglin, S Sarman, S Seregard, I van der Ploeg, A Kvanta; Reduced Choroidal Neovascularization in Matrix Metalloproteinase-2 (MMP-2) Deficient Mice . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1290.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose:Previous studies have suggested a role for matrix metalloproteinase-2 (MMP-2) during angiogenesis including choroidal neovascularization (CNV) (Steen et al. IOVS.1998;39:2194-2200). To further address this we have compared the extent of experimental CNV in wild-type and MMP-2 knock-out mice. Methods:Experimental CNV was induced in C57 black wild type and MMP-2 knock-out mice using krypton laser photocoagulation of the fundus. Ten days after treatment CNV formation was evaluated using hematoxylin-eosin straining of serial sections. The maximal height of the CNV lesions was calculated using image analysis of digitalized histologic images. Expression of MMP-2 mRNA was determined by in situ hybridisation using anti-sense and sense cRNA probes. Results:MMP-2 mRNA expression was detected in the CNV lesions at day 3 after laser treatment and peaked at day 7 to 10 wherafter it slowly declined. The CNV lesions showed a reduced thickness in MMP-2 knock-out mice compared to wild type mice (p= 0.0066). Conclusion:The present study demonstrates that MMP-2 mRNA is expressed during experimental CNV in the mouse. The statistically significant difference between wild-type and MMP-2 knock-out mice shows that MMP-2 is involved in the formation of laser-induced CNV in the mouse.

Keywords: 346 choroid: neovascularization • 530 proteolysis • 606 transgenics/knock-outs 
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