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LC Berglin, S Sarman, S Seregard, I van der Ploeg, A Kvanta; Reduced Choroidal Neovascularization in Matrix Metalloproteinase-2 (MMP-2) Deficient Mice . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1290.
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Purpose:Previous studies have suggested a role for matrix metalloproteinase-2 (MMP-2) during angiogenesis including choroidal neovascularization (CNV) (Steen et al. IOVS.1998;39:2194-2200). To further address this we have compared the extent of experimental CNV in wild-type and MMP-2 knock-out mice. Methods:Experimental CNV was induced in C57 black wild type and MMP-2 knock-out mice using krypton laser photocoagulation of the fundus. Ten days after treatment CNV formation was evaluated using hematoxylin-eosin straining of serial sections. The maximal height of the CNV lesions was calculated using image analysis of digitalized histologic images. Expression of MMP-2 mRNA was determined by in situ hybridisation using anti-sense and sense cRNA probes. Results:MMP-2 mRNA expression was detected in the CNV lesions at day 3 after laser treatment and peaked at day 7 to 10 wherafter it slowly declined. The CNV lesions showed a reduced thickness in MMP-2 knock-out mice compared to wild type mice (p= 0.0066). Conclusion:The present study demonstrates that MMP-2 mRNA is expressed during experimental CNV in the mouse. The statistically significant difference between wild-type and MMP-2 knock-out mice shows that MMP-2 is involved in the formation of laser-induced CNV in the mouse.
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