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PS Bora, JH Sohn, SG Kang, NS Bora, HJ Kaplan; Role of Complement System in Choroidal Neovascularization . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1291.
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Purpose: To investigate the role of the complement system in the mouse model of laser-induced choroidal neovascularization (CNV). Methods: CNV was induced by laser photocoagulation in the C57B6 mouse with the krypton red laser (0.05 sec., 100 uM, 250 mW). Four laser spots were placed in each eye of three different groups of mice (n=5): Cobra Venom Factor(CVF) treated, C3 deficient and wild-type (control). Mice were perfused with 1ml of 3% dextran(molecular weight 2 million Daltons) and sacrificed on day 7. Their eyes were enucleated, fixed (1h in10% phosphate buffered formalin) and flat mounts stained with an anti-elastin (Cy-3 conjugated) antibody. The incidence and size of CNV was determined by confocal microscopy. Results: Triplicate experiments revealed the following incidence of CNV: control group = 97%; CVF treated group = 5%; C3 deficient group = 10%. Additionally, the CNV complex in each of the experimental groups was markedly smaller than that in the control, wild-type group. Conclusion: The mouse model of laser-induced CNV is dependent on the complement system for angiogenesis. The specific role played by the complement system in choroidal endothelial cell proliferation and CNV is being studied.
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