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LM Le, V Poulaki, A Lappas, B Kirchhof, AM Joussen; Activin A and VEGF are Coregulated in Choriodal Neovascular Membranes . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1302.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:Activin A is a dimeric glycoprotein that belongs to the transforming growth factor beta (TGF-beta) superfamily and is involved in the pathogenesis of proliferative membrane formation in both ischemic and nonischemic vitreoretinal diseases. We examined the protein levels and localization of activin A and VEGF in epiretinal and preretinal neovascular membranes. Methods:In 36 Patients with age related macular degeneration, CNV were excised by pars plana-vitrectomy. Membranes were snap frozen in liquid nitrogen or processed for embedding in paraffin. Activin A and VEGF choroidal protein levels were measured with an ELISA assay. Tissue sections were stained for CD31, VEGF and Activin A. Results:The total protein levels of activin A in the membranes correlated with the total VEGF protein levels. CD31 positive vascular endothelial cells as well as RPE cells were positive for VEGF and activin A with immunohistochemistry. The fibrotic areas of the vascular membranes were negative for activin A and VEGF. Conclusion:: Activin A is a potential autocrine and/or paracrine regulator of the VEGF expression in RPE and endothelial cells. Given the established role of VEGF in the formation of the CNV membranes, we can conclude that activin A plays a role in the pathogenesis of choroidal neovascularization by regulating the expression of VEGF.
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