December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Connective Tissue Growth Factor expression in choroidal neovascular membranes and its regulation by VEGF
S He; M Jin; V Woerpel; Y Sekine; SJ Ryan; DR Hinton
Author Affiliations & Notes
  • S He
    Department of Pathology Univ of Southern California Los Angeles CA
  • M Jin
    Department of Pathology Univ of Southern California Los Angeles CA
  • V Woerpel
    Doheny Eye Institute Los Angeles CA
  • Y Sekine
    Doheny Eye Institute Los Angeles CA
  • SJ Ryan
    Doheny Eye Institute Los Angeles CA
  • DR Hinton
    Department of Pathology Univ of Southern California Los Angeles CA
  • Footnotes
    Commercial Relationships   S. He, None; M. Jin, None; V. Woerpel, None; Y. Sekine, None; S.J. Ryan, None; D.R. Hinton, None. Grant Identification: Supported by grants: EY03040, EY01545; Rearch to Prevent Blindness; Fight for Sight and Arnold and M
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 1303. doi:
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      S He, M Jin, V Woerpel, Y Sekine, SJ Ryan, DR Hinton; Connective Tissue Growth Factor expression in choroidal neovascular membranes and its regulation by VEGF . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1303.

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Abstract

Abstract: : Purpose: To evaluate the expression of connective tissue growth factor (CTGF) in choroidal neovascular membranes (CNVM) of patients with age-related macular degeneration (AMD), the regulation of CTGF in vitro by vascular endothelial growth factor (VEGF) and the effect of CTGF on choroidal endothelial cell (CEC) function. Methods: Using immunohistochemical methods, we analyzed CTGF expression in ten surgically excised AMD-related CNVMs. The effects of CTGF on attachment, migration, proliferation and tube formation of bovine CEC were studied in cultures stimulated with CTGF. The effect of VEGF on the expression of CTGF protein was evaluated using Western blot. Results: Vascularized human CNVM showed strong CTGF immunoreactivity. Double staining revealed co-localization of CTGF with RPE cells and CEC. CTGF induced a significant increase in attachment, migration and tube formation in CEC. CTGF protein was upregulated in CEC by stimulation of VEGF. Conclusion: The expression of CTGF in human CNVM and its upregulation by VEGF suggest that CTGF may play a role in the pathogenesis of AMD-related CNV.

Keywords: 308 age-related macular degeneration • 346 choroid: neovascularization • 423 growth factors/growth factor receptors 
© 2002, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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