December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Evaluation And Comparision Of Vitreal Ngf Levels In Human Proliferative Diabetic Retinopathy And Proliferative Vitreoretinopathy
Author Affiliations & Notes
  • KV Chalam
    Department of Ophthalmology University of Florida Jacksonville FL
  • N Agarwal
    Department of Cell biology and Anatomy University of North Texas Fortworth TX
  • R Agarwal
    Department of Cell biology and Anatomy University of North Texas Fortworth TX
  • R Wordinger
    Department of Cell biology and Anatomy University of North Texas Fortworth TX
  • S Vinjamaram
    Department of Ophthalmology University of Florida Jacksonville FL
  • Footnotes
    Commercial Relationships   K.V. Chalam, None; N. Agarwal, None; R. Agarwal, None; R. Wordinger, None; S. Vinjamaram, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 1310. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      KV Chalam, N Agarwal, R Agarwal, R Wordinger, S Vinjamaram; Evaluation And Comparision Of Vitreal Ngf Levels In Human Proliferative Diabetic Retinopathy And Proliferative Vitreoretinopathy . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1310.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose: To evaluate and compare Nerve Growth Factor (NGF) vitreal levels in human Proliferative Diabetic Retinopathy (PDR) and Proliferative Vitreoretinopathy (PVR). Methods: Nineteen patients were selected for the study and vitreous samples were obtained during surgery. 4 patients had proliferative vitreoretinopathy and 5 patients had proliferative diabetic retinopathy. Epiretinal membranes (ERM) were obtained from 10 patients to be used as controls. ELISA, a sensitive technique for accurately determining the amount of protein in a given sample by means of an enzyme-catalyzed color change was used to determine the amount of Nerve Growth factor in each vitreous sample by comparing against a standard curve to obtain the concentration f NGF protein in the sample. Results: NGF level was significantly elevated in the PDR samples as compared to the PVR samples and controls. The PVR samples had a mean NGF concentration of 145.58 pg/ml, PDR samples had a mean NGF concentration of 1544.56 pg/ml and the control ERM samples had a concentration of 44.28 pg/ml. The median NGF concentration in PVR and control samples was 109.08 and 32.65 respectively as opposed to a median of 1903.35 in PDR samples. NGF was low or undetectable in 2/10 controls. Kruskall Wallis and Dunn's statsitical tests revealed a statistically significant difference (p<0.05) among the three groups. Conclusion: Patients with proliferative diabetic retinopathy and vitreoretinopathy have increased levels of vitreal Nerve growth factor. Several studies have shown raised NGF levels to be associated with hypoxia. Chronic hypoxia seen in PDR may be one of the factors for the increased NGF levels observed in PDR. Following studies on a larger sample population, NGF may be used, to screen and determine the severity of proliferative diabetic retinopathy.

Keywords: 388 diabetic retinopathy • 524 proliferative vitreoretinopathy • 628 vitreoretinal surgery 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×