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H Morita, K Tamai, Y Matsuda, A Matsubara, Y Niwa, K Tomida, Y Ogura; Gliclazid Inhibits Leukocyte Entrapment in the Retinal Microcirculation of Diabetic Rats . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1324.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: It has been reported that leukocyte entrapment is increased in the diabetic retina. We reported that oral administration of gliclazide, an oral hypoglycemic agent, which has free radical scavenger property, decreased leukocyte entrapment in the retinal microcirculation during early diabetic period (ARVO, 2001). This study was designed to evaluate the dosage dependant effect of gliclazid on leukocyte entrapment in the retinal microcirculation of diabetic rats. Methods: Diabetes was induced in male Brown-Norway rats by intravenous injection of streptozotocin (60mg/kg). Diabetic rats were divided into 4 groups. Group A received no treatment. Group B received 0.01% gliclazid mixed with food during a 7-week diabetic period. Group C received 0.1% gliclazid, and Group D received 1% gliclazid, respectively. Non-diabetic rats were used as control. Leukocyte entrapment in the retinal microcirculation was quantitatively evaluated in vivo with acridine orange digital fluorography. Results: Blood glucose levels in all diabetic rats were significantly increased compared with control, but were not different among the 4 groups. The number of leukocytes trapped in the retinal microcirculation at 30 minutes after dye injection was significantly greater in group A than in control (24.12.9, 6.00.5cells/mm2, respectively, p<0.01). Compared with group A, the number of trapped leukocytes decreased significantly in group C (15.42.0 cells/mm2, p<0.01). and especially in group D (11.12.6 cells/mm2, p<0.01). However, no significant difference was seen in group B (21.61.7 cells/mm2). Conclusion: Oral administration of gliclazide decreased the enhanced leukocyte entrapment in the retinal microcirculation according to the dosage during early diabetic period. Gliclazid may have a potential that reduces retinal microvascular disturbance in early diabetes.
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