December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Comparison of Retina Specific Amine Oxidase (AOC2) and Super Family Gene Vascular Adhesion Protein 1 (AOC3) Tandemly Located in Chromosome 17q21
Author Affiliations & Notes
  • K Izumi
    National Center for Sensory Organs National Tokyo Medical Center Tokyo Japan
  • Q Zhang
    National Center for Sensory Organs National Tokyo Medical Center Tokyo Japan
  • Y Sergeev
    National Eye Institute National Insitute of Health Rockville MD
  • Y Mashima
    Department of Ophthalmology
    Keio University School of Medicine Tokyo Japan
  • S Noda
    Department of Nursing Tokai University School of Health Science Isehara Japan
  • Y Imamura
    Department of Ophthalmology
    Keio University School of Medicine Tokyo Japan
  • J Kudoh
    Department of Molecular Biology
    Keio University School of Medicine Tokyo Japan
  • N Shimizu
    Department of Molecular Biology
    Keio University School of Medicine Tokyo Japan
  • Y Tanaka
    National Center for Sensory Organs National Tokyo Medical Center Tokyo Japan
  • T Iwata
    National Center for Sensory Organs National Tokyo Medical Center Tokyo Japan
  • Footnotes
    Commercial Relationships   K. Izumi, None; Q. Zhang, None; Y. Sergeev, None; Y. Mashima, None; S. Noda, None; Y. Imamura, None; J. Kudoh, None; N. Shimizu, None; Y. Tanaka, None; T. Iwata, None. Grant Identification: Ministry of Health, Labor and Welfare, Ministry of Education, Culture, Sports, Science, JAPAN
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 1433. doi:
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    • Get Citation

      K Izumi, Q Zhang, Y Sergeev, Y Mashima, S Noda, Y Imamura, J Kudoh, N Shimizu, Y Tanaka, T Iwata; Comparison of Retina Specific Amine Oxidase (AOC2) and Super Family Gene Vascular Adhesion Protein 1 (AOC3) Tandemly Located in Chromosome 17q21 . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1433.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To functionaly compare retina specific amine oxidase (AOC2) with super family gene vascular adhesion protein 1 (AOC3). Methods: The mouse retina specific amine oxidase (AOC2) previously cloned by cDNA library screening and 5'/3'-RACE method was used as probe to screen mouse genomic library. The entire genomic structure was deterimed and the 5' and 3' regions beyond the AOC2 gene were also sequenced. AOC2 promoter sequence was analyzed with trascription factor database and compared with AOC3 promoter. The coding sequence of mouse AOC2 cDNA was cloned into expression vectors (pMIB/V5-His, Invitrogen, Inc.) for Sf21 insect cell expression. Purified protein was tested for diamine oxidase acitivty using putrescine as substrate. The 3D structure of AOC2 protein was created by 3D modeling software based on bacteria amine oxidase crystal structure and compared with AOC3. Results: The AOC2, 4,625 bp in legnth, is composed of 4 exons and 3 introns following the canonical donor-acceptor rule. One of the super family of amine oxidase, vascular adhesion protein 1 (AOC3), was tandemly located approximately 1.4 Kb downstream of AOC2 exon 4. This was also confirmed in human gene structure of AOC2 and AOC3. Promoter sequence comparison of AOC2 and AOC3 revealed significantly low similarity (10%) but several short homologous stretch of sequences matching but in different order. Comparison of AOC2 and AOC3 protein structure showed posible functional similarity. Conclusion: Super family of aime oxidase AOC2 and AOC3 was tandemly located approximately 1.4 Kb apart. Enzyme activity and 3D structure analysis show functional similarity but different tissue specificity.

Keywords: 415 ganglion cells • 557 retina: proximal(bipolar, amacrine, and ganglion cells) • 476 molecular biology 
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