December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Macrophage Migration Inhibitory Factor (MIF) Enhances Th1 Type Cytokine in the Human Eye
Author Affiliations & Notes
  • H Takase
    Department of Ophthalmology & Visual Science Tokyo Medical and Dental University Graduate School Tokyo Japan
  • S Sugita
    Department of Ophthalmology & Visual Science Tokyo Medical and Dental University Graduate School Tokyo Japan
  • DJ Rhee
    Laboratory of Mechanisms of Ocular Disease National Eye Institute National Institutes of Health Bethesda MD
  • Y Imai
    Department of Ophthalmology & Visual Science Tokyo Medical and Dental University Graduate School Tokyo Japan
  • C Taguchi
    Department of Ophthalmology Kurume University School of Medicine Fukuoka Japan
  • Y Sugamoto
    Department of Ophthalmology & Visual Science Tokyo Medical and Dental University Graduate School Tokyo Japan
  • Y Tagawa
    Department of Ophthalmology
    Hokkaido University School of Medicine Hokkaido Japan
  • J Nishihira
    Central Research Institute
    Hokkaido University School of Medicine Hokkaido Japan
  • P Russell
    Laboratory of Mechanisms of Ocular Disease National Eye Institute National Institutes of Health Bethesda MD
  • M Mochizuki
    Department of Ophthalmology & Visual Science Tokyo Medical and Dental University Graduate School Tokyo Japan
  • Footnotes
    Commercial Relationships   H. Takase, None; S. Sugita, None; D.J. Rhee, None; Y. Imai, None; C. Taguchi, None; Y. Sugamoto, None; Y. Tagawa, None; J. Nishihira, None; P. Russell, None; M. Mochizuki, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 1564. doi:
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    • Get Citation

      H Takase, S Sugita, DJ Rhee, Y Imai, C Taguchi, Y Sugamoto, Y Tagawa, J Nishihira, P Russell, M Mochizuki; Macrophage Migration Inhibitory Factor (MIF) Enhances Th1 Type Cytokine in the Human Eye . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1564.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To identify the expression and secretion of macrophage migration inhibitory factor (MIF) in human trabecular meshwork (HTM) and evaluate its role in ocular inflammation. Methods: The tissue samples of HTM cells were isolated from donor human eyes or corneoscleral buttons, and the HTM cells were cultured. The expression of MIF on HTM cells was evaluated by RT-PCR, immunofluorescence microscopy, western blotting and ELISA. T cell clones (TCCs) were established from ocular infiltrating cells of patients with uveitis. The pathological role of MIF was evaluated in terms of regulatory effects on cytokine production by T cells using ELISA. Results: MIF was detected in the HTM at mRNA and protein levels. MIF was also shown to be secreted by the HTM cells in culture by an ELISA assay. The HTM supernatant enhanced IFN-γ production, but not IL-10, by TCCs, and these effects were neutralized by anti-MIF antibodies. Similarly, recombinant MIF enhanced the IFN-γ production by the TCCs. Conclusion: MIF is expressed and secreted in the human trabecular meshwork, and MIF has the capacity to enhance Th1 cytokines and may play a role as an inflammatory cytokine in the eye.

Keywords: 601 trabecular meshwork • 380 cytokines/chemokines • 612 uveitis-clinical/animal model 
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