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H Takase, S Sugita, DJ Rhee, Y Imai, C Taguchi, Y Sugamoto, Y Tagawa, J Nishihira, P Russell, M Mochizuki; Macrophage Migration Inhibitory Factor (MIF) Enhances Th1 Type Cytokine in the Human Eye . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1564.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: To identify the expression and secretion of macrophage migration inhibitory factor (MIF) in human trabecular meshwork (HTM) and evaluate its role in ocular inflammation. Methods: The tissue samples of HTM cells were isolated from donor human eyes or corneoscleral buttons, and the HTM cells were cultured. The expression of MIF on HTM cells was evaluated by RT-PCR, immunofluorescence microscopy, western blotting and ELISA. T cell clones (TCCs) were established from ocular infiltrating cells of patients with uveitis. The pathological role of MIF was evaluated in terms of regulatory effects on cytokine production by T cells using ELISA. Results: MIF was detected in the HTM at mRNA and protein levels. MIF was also shown to be secreted by the HTM cells in culture by an ELISA assay. The HTM supernatant enhanced IFN-γ production, but not IL-10, by TCCs, and these effects were neutralized by anti-MIF antibodies. Similarly, recombinant MIF enhanced the IFN-γ production by the TCCs. Conclusion: MIF is expressed and secreted in the human trabecular meshwork, and MIF has the capacity to enhance Th1 cytokines and may play a role as an inflammatory cytokine in the eye.
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