December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Cytokine Production by Human Corneal Endothelial Cells and Keratocytes, and Their Effects on Ocular Infiltrating T Cells
Author Affiliations & Notes
  • S Sasaki
    Department of Ophthalmology & Visual Science Tokyo Medical and Dental University Graduate School Tokyo Japan
  • H Takase
    Department of Ophthalmology & Visual Science Tokyo Medical and Dental University Graduate School Tokyo Japan
  • Y Nagai
    Miyata Eye Hospital Miyazaki Japan
  • Y Imai
    Department of Ophthalmology & Visual Science Tokyo Medical and Dental University Graduate School Tokyo Japan
  • K Kamoi
    Department of Ophthalmology & Visual Science Tokyo Medical and Dental University Graduate School Tokyo Japan
  • S Sugita
    Department of Ophthalmology & Visual Science Tokyo Medical and Dental University Graduate School Tokyo Japan
  • K Miyata
    Miyata Eye Hospital Miyazaki Japan
  • Y Tagawa
    Department of Ophthalmology
    Hokkaido University Graduate School of Medicine Hokkaido Japan
  • J Nishihira
    Central Research Institute
    Hokkaido University Graduate School of Medicine Hokkaido Japan
  • M Mochizuki
    Department of Ophthalmology & Visual Science Tokyo Medical and Dental University Graduate School Tokyo Japan
  • Footnotes
    Commercial Relationships   S. Sasaki, None; H. Takase, None; Y. Nagai, None; Y. Imai, None; K. Kamoi, None; S. Sugita, None; K. Miyata, None; Y. Tagawa, None; J. Nishihira, None; M. Mochizuki, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 1566. doi:
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    • Get Citation

      S Sasaki, H Takase, Y Nagai, Y Imai, K Kamoi, S Sugita, K Miyata, Y Tagawa, J Nishihira, M Mochizuki; Cytokine Production by Human Corneal Endothelial Cells and Keratocytes, and Their Effects on Ocular Infiltrating T Cells . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1566.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To evaluate cytokine production by human corneal endothelial cells (EC) and keratocytes (KC) and their effects on ocular infiltrating T cells in order to investigate the role of corneal EC and KC in the immunoregulation of the anterior chamber. Methods: The tissue samples of EC and KC were isolated from eye bank eyes and cultured in DMEM containing 10%FBS. Cytokines in the culture were measured by ELISA (TGF-ß2, interleukin (IL)-12, MIF) and cytometric bead array kit (IFN-γ, TNF-α, IL-2, IL-4, IL-5 and IL-10). T cell clones (TCCs) were established from ocular infiltrating cells of a patient with HTLV-I uveitis. The pathological roles of EC and KC were evaluated in terms of regulatory effects on IFN-γ production by the TCCs. Results: MIF was detected in each supernatant of EC (1.80 ± 1.18ng/ml) and KC (1.93 ± 0.85ng/ml). TGF-ß2, IFN-γ, TNF-α, IL-2, IL-4, IL-5 and IL-10 were not detected in the supernatants of EC and KC. The supernatants of both EC and KC enhanced IFN-γ production by TCCs in a dose-dependent manner. Conclusion: Corneal endothelial cells and keratocytes secrete MIF, and that may enhance IFN-γ production by ocular infiltrating T cells.

Keywords: 371 cornea: endothelium • 374 cornea: stroma and keratocytes • 380 cytokines/chemokines 
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