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L Kodjikian, C Chanlois, V Bostvironnois, E Mari, J Freney, T Roger, C Burillon; In Vivo Study of Bacterial Adherence to Five Types of Intraocular Lenses . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1589.
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Purpose: The aim of this study was to determine in vivo the adherence of Staphylococcus epidermidis (ATCC 14990) to 120 intraocular lenses made of five different biomaterials: fluorine polymethylmethacrylate (PMMA), heparinized PMMA, silicone, hydrophobic acrylic and hydrogel. The pig was chosen as an animal model in endophthalmitis, after a bibliographical analysis and a personal study on its aqueous humor composition. Methods: The IOLs, previously infected, were implanted into 90 domestic pigs, after realizing the removal of the crystalline lens under aseptic conditions. The euthanasia of the animals was made at 24 hours, 72 hours and 1 week. The extent of bacterial binding was measured by counting. The results were compared using a two-factors variance analysis. Results: The bound bacteria per area unit were found in increasing order from hydrogel, fluorine PMMA, hydrophobic acrylic, heparinized PMMA, and finally to the silicone polymer. Only the difference between hydrogel and fluorine PMMA was not statistically significant. Conclusion: Bacterial adherence to the implant surface must therefore depend on the hydrophobicity or hydrophilicity of the biomaterial. This classification is similar to those obtained in a previously in vitro study (Burillon et al. 2002).1 Adhesion also depends on the nature of the surrounding medium. This medium is very difficult to modelize considering its complexicity and thus makes in vivo study essential. 1 Burillon C, Kodjikian L, Pellon G, Martra A, Freney J, Renaud FNR. "in-vitro study of bacterial adherence to different types of intraocular lenses" Drug Development and industrial pharmacy. 2002; 28(1): 95-99
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