December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Detection of Common Ocular Pathogens Using Fluorescence Spectroscopy
DV Will; RB Rosen; M Shah; A Katz; H Savage
Author Affiliations & Notes
  • DV Will
    Ophthalmology The New York Eye and Ear Infirmary New York NY
  • RB Rosen
    Ophthalmology The New York Eye and Ear Infirmary New York NY
  • M Shah
    Ophthalmology The New York Eye and Ear Infirmary New York NY
  • A Katz
    City University of New York New York NY
  • H Savage
    Ophthalmology The New York Eye and Ear Infirmary New York NY
  • Footnotes
    Commercial Relationships   D.V. Will, None; R.B. Rosen, None; M. Shah, None; A. Katz, None; H. Savage, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 1614. doi:
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      DV Will, RB Rosen, M Shah, A Katz, H Savage; Detection of Common Ocular Pathogens Using Fluorescence Spectroscopy . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1614.

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Abstract

Abstract: : Purpose:To evaluate a potentially noninvasive method of detecting bacteria and common ocular pathogens using fluorescence spectroscopy. Methods:Emission, excitation, and synchronous fluorescent scans of several common ocular pathogens including Pseudomonas, Staph. Aureus, E. coli, and Bacillus subtilis suspended in normal saline at an optical density of approximately 1.00 were performed. Data was graphed illustrating the intensity of the fluorescence stimulated by the wavelength of excitation and compared. Results:Each of the tested pathogens demonstrated a "fingerprint" pattern of fluorescence intensity peaks at particular wavelengths. The patterns of the peaks were sufficient to differentiate the organisms. Conclusion:Bacteria in solution can be identified by their characteristic peaks using fluorescence spectroscopy. This technology may allow for rapid detection of intraocular infections noninvasively. It may prove to be a useful means of rapidly identifying bacteria in both clinical ophthalmology and in other health care situations, including possibly identifying potential biological weapons such as Bacillus species.

Keywords: 469 microbial pathogenesis: experimental studies • 432 imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • 358 clinical laboratory testing 
© 2002, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2015 Association for Research in Vision and Ophthalmology.
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