December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
The Limbal Stem Cell Deficiency in Mice that are Heterozygous Null for Pax6
Author Affiliations & Notes
  • JM Collinson
    Department of Reproductive and Developmental Sciences University of Edinburgh Edinburgh United Kingdom
  • RE Hill
    Comparative and Developmental Genetics Section MRC Human Genetics Unit Edinburgh United Kingdom
  • JD West
    Department of Reproductive and Developmental Sciences University of Edinburgh Edinburgh United Kingdom
  • Footnotes
    Commercial Relationships   J.M. Collinson, None; R.E. Hill, None; J.D. West, None. Grant Identification: Support: Wellcome Trust
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 1629. doi:
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      JM Collinson, RE Hill, JD West; The Limbal Stem Cell Deficiency in Mice that are Heterozygous Null for Pax6 . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1629.

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Abstract

Abstract: : Purpose: To investigate possible autonomous deficiencies of corneal epithelial cells in small eye mice (heterozygous null for Pax6). Methods: Aggregation chimeras were made between wildtype 8-cell embryos and small eye embryos that carried a constitutively expressed LacZ transgene. The chimeric mice were grown to adulthood and the pattern of chimerism (revealed by X-Gal staining of the limbal and corneal epithelium) was compared to control chimeras in which both the LacZ-positive and LacZ-negative cells were wildtype with respect to Pax6. Results: In chimeric mice that were a mixture of LacZ+ and LacZ- cells a pattern of blue radial stripes was seen in the adult corneal epithelium that reflects clonal patterns of cell proliferation and migration during corneal maintenance. The blue stripes that were produced by Pax6+/- cells in chimeras were qualitatively identical to those produced by wild-type cells in control chimeras. Cells that are heterozygous for Pax6 can populate the corneal epithelium and be maintained normally into adulthood in chimeric eyes providing the lens is wildtype. Conclusion: We suggest that deficiencies in the corneal epithelium of small eye mice may be, at least in part, non-autonomous, and are exacerbated by the disruption of anterior segment development that results from having a mutant lens

Keywords: 372 cornea: epithelium • 370 cornea: basic science • 316 animal model 
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