December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Effects of Steroids on Mucins of Human Corneal Epithelial Cells
Author Affiliations & Notes
  • KY Seo
    Ophthalmology Inst Vis Res Yonsei Univ Med Coll Seoul Republic of Korea
  • JW Park
    Taegu Yonsei Eye Clinic Taegu Republic of Korea
  • SE Jung
    Ophthalmology Inst Vis Res Yonsei Univ Med Coll Seoul Republic of Korea
  • SJ Lee
    Ophthalmology Inst Vis Res Yonsei Univ Med Coll Seoul Republic of Korea
  • JH Lee
    Ophthalmology Inst Vis Res Yonsei Univ Med Coll Seoul Republic of Korea
  • JB Lee
    Ophthalmology Inst Vis Res Yonsei Univ Med Coll Seoul Republic of Korea
  • EK Kim
    Ophthalmology Inst Vis Res Brain Korea 21 Division of Medical Sciences Yonsei Univ Med Coll Seoul Republic of Korea
  • Footnotes
    Commercial Relationships   K.Y. Seo, None; J.W. Park, None; S.E. Jung, None; S.J. Lee, None; J.H. Lee, None; J.B. Lee, None; E.K. Kim, None. Grant Identification: Support: Korea Research Foundation Grant KRF-2000-041-F00246
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 1642. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      KY Seo, JW Park, SE Jung, SJ Lee, JH Lee, JB Lee, EK Kim; Effects of Steroids on Mucins of Human Corneal Epithelial Cells . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1642.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Abstract: : Purpose:To examine whether mucin gene expression is influenced by steroid hormones in cultured human corneal epithelial cells (HCECs). The effects of dexamethasone on the expression of MUC1 and MUC4, two known mucins produced by corneal epithelial cells were determined. Methods:HCECs were cultured in medium supplemented with dexamethasone. The modulations of MUC1 and MUC4 expression by dexamethasone were investigated by RT-PCR and Western blot analysis. Results:The expression of MUC1 mRNA and its protein were enhanced in HCECs by dexamethasone. However, treatment of HCECs with dexamethasone caused a decrease in MUC4 mRNA and its protein. The effects of dexamethasone were abolished by a glucocorticoid antagonist (RU486). Conclusion:Steroid hormones appear to be implicated in the expression of mucin in HCECs. This study supports this role of glucocorticoid and furthermore suggests that glucocorticoid receptors may be an important way in the induction of its expression. Thus, steroids could be a component of the process that causes the modulation of mucin in corneal epithelial cells.

Keywords: 376 cornea: tears/tear film/dry eye • 421 glycoconjugates/glycoproteins • 377 corticosteroids 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×