December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Regulation of Growth and MMP's Expression by Corneal Epithelial Cells Expanded on Amniotic Membrane
Author Affiliations & Notes
  • JC Rigal-Sastourne
    Ophthalmology AIH Val de Grace Paris France
  • JM Tixier
    Biotechnology and Eye Laboratory Paris France
  • M Savoldelli
    Biotechnology and Eye Laboratory Paris France
  • F Dagonnet
    Biotechnology and Eye Laboratory Paris France
  • JP Renard
    Ophthalmology AIH Val de Grace Paris France
  • JF Maurin
    Ophthalmology AIH Val de Grace Paris France
  • G Renard
    Ophthalmology Hotel Dieu Hospital Paris France
  • JM Legeais
    Biotechnology and Eye Laboratory Paris France
  • Footnotes
    Commercial Relationships   J.C. Rigal-Sastourne, None; J.M. Tixier, None; M. Savoldelli, None; F. Dagonnet, None; J.P. Renard, None; J.F. Maurin, None; G. Renard, None; J.M. Legeais, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 1688. doi:
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    • Get Citation

      JC Rigal-Sastourne, JM Tixier, M Savoldelli, F Dagonnet, JP Renard, JF Maurin, G Renard, JM Legeais; Regulation of Growth and MMP's Expression by Corneal Epithelial Cells Expanded on Amniotic Membrane . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1688.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To compare cellular growth and expression of matrix metalloproteases MMP-2 and MMP-9 by cultured epithelial corneal cells on treated amniotic membrane. Methods: Acrylamid gel zymography were performed to compare semi quantitatively MMP's expression by cultured corneal epithelial cells on differents specimen of amniotic membrane. Human limbal explants were cultured in vitro on amniotic membrane using airlifting technique. Amniotic membrane was chemically pre-treated to scratch epithelial monolayer. The culture on acellular amniotic membrane was compared with culture on cellular amniotic membrane. Results: Expanded corneal epithelial cells on acellular membrane formed a confluent epithelium layer in 2 weeks. Epithelial outgrowth was more rapid to confluence on acellular than cellular membrane (p<0.05). Zymography showed MMP's overexpression in cellular amniotic membrane than acellular amniotic membrane when epithelial cells were not a confluent layer. Conclusion: These results correlate the MMP's expression with the cultured epithelium corneal outgrowth on amniotic membrane to support that corneal epithelial cells can be cultured more easily to confluence on acellular amniotic membrane.

Keywords: 372 cornea: epithelium • 474 microscopy: light/fluorescence/immunohistochemistry • 399 enzymes/enzyme inhibitors 
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