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C-C Wu, S-J Chen, J-H Liu, S-M Lee, W-M Hsu; Quantification of Intraocular Endostatin; A Newly Identified Angiogenesis Inhibitor . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1750.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose:Endostatin is a newly identified angiogenic inhibitor. The aims of the study are to quantify normal human intraocular endostatin and to compare aqueous levels between normal and herpetic keratitis with corneal neovascularization. Methods:Aqueous(N=17)and serum(N=10)samples were collected from patients during cataract operations. Twelve control cases were senile cataract and five cases had corneal neovascularization due to old herpetic keratitis. Undiluted vitreous samples(N=13) were collected during vitrectomy from macular pucker(N=4), macular hole(N=4), Coats' disease(N=1), and cataract operation complicated with vitreous loss(N=4). Samples were stored then quantified by ELISA technique. Results:The average level of endostatin was 24+ 10.5 ng/ml in serum. The average level of endostatin was 8.9+2.5 ng/ml in normal aqueous and 7.4+1.0 ng/ml in aqueous with corneal neovascularization. They were not significantly different(Mann-Whitney U test, p=0.1). As the level in vitreous from Coats' disease was too high(159.9 ng/ml),so this data was excluded from the study for the normal average. The average level in vitreous(N=12) was 15.0+8.5 ng/ml, and was higher than aqueous level(Mann-Whitney U test, p<0.01). Both aqueous and vitreous level were lower than serum(Mann-Whitney U test, p<0.01 and p=0.01,respectively). Conclusion:Eye contains at least two kinds of endogenous angiogenic inhibitors, pigment epithelium-derived factor(PEDF) and endostatin. Only endostatin can not explain the avascularity of vitreous. Aqueous endostatin was not significantly related to corneal neovascularization in our study. Angiogenesis should be modulated by multiple angiogenic factors and multiple inhibitors. Besides anti-angionenic effect, endostatin can be an indicator of pathological changes of vessels such as telangiectasis in Coats' disease. Endostatin is multi-functional just like PEDF.
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