December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
Pharmacological Analysis of the Rat Cone Electroretinogram
Author Affiliations & Notes
  • L Xu
    Cole Eye Institute Cleveland Clinic Foundation Cleveland OH
  • SL Ball
    Research Service Cleveland VAMC Cleveland OH
  • KR Alexander
    Department of Ophthalmology & Visual Sciences University of Illinois at Chicago College of Medicine Chicago IL
  • NS Peachey
    Cole Eye Institute Cleveland Clinic Foundation Cleveland OH
  • Footnotes
    Commercial Relationships   L. Xu, None; S.L. Ball, None; K.R. Alexander, None; N.S. Peachey, None. Grant Identification: Support: VA
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 1814. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      L Xu, SL Ball, KR Alexander, NS Peachey; Pharmacological Analysis of the Rat Cone Electroretinogram . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1814.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Abstract: : Purpose: The electroretinogram (ERG) of the rat cone system has proven useful for evaluating experimental treatments for retinal disease. However, the relative contributions of the different retinal cell types to the rat cone ERG have not been determined. Here, we address this issue using a pharmacological approach. Methods: ERGs were recorded from Sprague-Dawley rats under ketamine/xylazine anesthesia. Pharmacological agents dissolved in saline were injected into the vitreous, and cone ERGs were recorded approximately 2 hours later. In different experiments, we used 2-amino-4-phosphonobutyric acid (L-AP4), sodium aspartate (ASP), piperidine dicarboxylic acid (PDA) or vehicle (saline) alone. Stimulus flashes of varying durations were superimposed upon a steady rod-desensitizing adapting field. Results: In response to light, rat cone photoreceptor activity, isolated by treatment with ASP or L-AP4+PDA, generated an ERG component of negative polarity that was substantially smaller than the human cone photoreceptor response under identical recording conditions. The response of the cone DBCs, estimated by subtraction of L-AP4-treated responses from those obtained after saline injection, contributed a positive potential of large amplitude to the rat cone ERG. In comparison, the response of the HBCs, estimated by subtracting responses recorded after PDA from those obtained after saline injection, and by subtracting responses recorded after L-AP4 from those obtained after ASP, was of small amplitude, with a negative polarity at stimulus onset and a positive polarity at stimulus offset. Conclusion: The relative contributions of the DBC- and HBC- driven responses to the rat cone ERG are distinctly different from those underlying the primate ERG response (Sieving et al., Vis Neurosci 1994;11: 519-532). While DBCs and HBCs make a substantial contribution to the primate cone ERG, the rat cone ERG response is dominated by the DBC component, and HBCs make only a minor contribution.

Keywords: 396 electroretinography: non-clinical • 555 retina: distal(photoreceptors, horizontal cells, bipolar cells) • 514 pharmacology 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.