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E Sekiyama, S Kawasaki, K Endo, C Mochida, T Nakamura, S Kinoshita; Gene Expression Profiling of Angiogenic and Anti-Angiogenic Factors Between Corneal and Conjunctival Epithelial Cells . Invest. Ophthalmol. Vis. Sci. 2002;43(13):1865.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: Corneal avascularity is thought to be maintained by unknown factors produced by corneal epithelial cells and/or keratocytes. The purpose of this study is to elucidate the role of corneal epithelial cells in the maintenance of corneal avascularity. Methods: Human samples in this study included 4 fresh corneal buttons obtained at PKP surgery, 3 preserved corneas purchased from an US eye bank, 6 conjunctival epithelia obtained from normal volunteers by brush cytology, and 1 cultivated corneal epithelial sheet. RNAs extracted from epithelial cells of these samples were subjected to subsequent iAFLP analysis, a kind of competitive RT-PCR. Genes examined were angiogenic factors such as VEGF, angiogenin, IL-8, erythropoietin and TNF alpha 2, and anti-angiogenic factors such as angiogenin inhibitor, GRO-beta, IP-10, NK4 and thrombospondin 3. Results: The gene expression of VEGF, IL-8, and angiogenin, known as angiogenic factors, were markedly down-regulated in corneal and cultivated corneal samples with apparent segregation. Paradoxically, GRO-beta and angiogenin inhibitor, known as anti-angiogenic factors, were also down-regulated in corneal samples. Notably, VEGF, known to be a key factor in angiogenesis, was less expressed in fresh corneal and cultivated corneal epithelium than in fresh conjunctival epithelium, but remarkably up-regulated in preserved corneal epithelium. Conclusion: Down-regulation of several angiogenic and anti-angiogenic factors suggests complex interaction among these factors in precisely regulating corneal avascularity. View OriginalDownload SlideView OriginalDownload Slide
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