Abstract
Abstract: :
Purpose: To study the possibility that neurotrophic factor induces adult rat bone marrow stromal cells(BMSCs ) into neurons for further investigation of an alternative for neuron regeneration and the treatment of retinal neuropathy. Method: The bone marrow stromal cells from adult rat were isolated and cultured for 6 passages. The BMSCs were pretreated with DMEM/20%FBS/1mg/L bFGF for 24 hours. Then the media were replaced by induction media consisting of DMEM/50ng/ml NGF/50ng/ml BDNF. The morphological changes of the cells were observed under phase contrast microscope, the cells were stained immunocytochemically with anti-Nestin, -NF,-Map-2, -NSE and -GFAP antibodies. Results: After an hour of induction ,some cells showed morphological changes, five hours later, many BMSCs became neuron-like cells and were stained positively with Nestin, NF, Map-2,NSE antibody, but negatively with GFAP. Conclusion: The bone marrow stromal cells can differentiate into neuron- like cells by the induction of neurotrophic factor. Key words Bone marrow stromal cells Neurotrophic factor Induction neuron regeneration
Keywords: 489 neuroprotection • 340 cell-cell communication • 492 nutritional factors