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H Osawa, jW Streilein; Evidence That Mucosal Tolerance, Induced By Allogeneic Cornea Grafts, May Contribute To Graft Acceptance . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2272.
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Purpose:Active suppression of anti-donor immunity has been implicated in the high rate of acceptance and prolonged survival of orthotopic corneal allografts in mice. We investigated the extent to which corneal allografts placed subconjunctivally (a component of the mucosal immune system) inhibit acquisition of donor-specific delayed hypersensitivity. Methods:Fragments (3x1 mm) of C57BL/6 cornea or body wall skin were placed subconjunctivally (scon) or subcutaneously (sc) in normal BALB/c recipients. Acquisition of donor-specific delayed hypersensitivity (DH) was assessed at selected intervals post grafting, through 8 weeks. Suppressor cells were assessed by local adoptive transfer assays. Results:Mice that received allogeneic skin fragments scon or sc displayed donor-specific DH at 2 week post-grafting and maintained this reactivity for 8 weeks. Mice that received allogeneic cornea fragments scon also displayed donor-specific within 2 week of grafting, but thereafter, these mice lost this reactivity. Moreover, spleen and draining lymph node cells from mice bearing scon cornea grafts beyond 8 wk suppressed DH in local adoptive transfer assays. Conclusion:Allogeneic corneal tissue placed scon generates suppression of donor-specific DH (mucosal tolerance), raising the possibility that tolerance related to the mucosal immune system may contribute to the success of orthotopic corneal allografts.
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