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K Sedlakova, H Dannowski, C Brandt, N Schmidt, M Filipec, C Hartmann, T Ritter, U Pleyer; Adenovirus-Mediated Gene Transfer of vIL-10 in Experimental Corneal Transplantation . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2279.
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Purpose: Gene transfer of immunomodulatory cytokines appears to be a promising approach in corneal transplantation. The EBV-encoded IL-10 homologue (vIL-10) has prominent anti-inflammatory potential and is able to modulate immune responses away from TH-1 towards TH-2. Therefore we determined whether (Ad)-mediated gene transfer of this cytokine has the ability to influence corneal graft rejection in a MHC I/II mismatched rat model. Methods: Corneal grafts from Lewis and Wistar-Furth rats were transduced ex vivo with 1x108 pfu of E1-/E3- Ad5CMVvIL-10 (n=8) or E1- Ad5 dl 312 without transgene (n=8) and were transplanted into Lewis recipients. All allogeneic grafts were performed together with the syngeneic control. Nontransduced grafts (n=7) from Lewis and Wistar-Furth rats served as additional control. Results: All syngeneic grafts remained clear for the whole observation period. The mean survival time of Ad5CMVvIL-10 treated group (22.75±12.93 D) or Ad5dl312 treated group (22.86±8.03 D) was not significantly prolonged (p = 0.2583 and p = 0.1657 respectively) compared to untreated controls (17.43±4.2 D). Conclusion: vIL-10 in these experimental settings was not able to significantly delay graft rejection. Interestingly, transduction with the control-Ad did not accelerate but even showed the tendency to delay this process. Further studies will clarify the role of vIL-10 in allograft rejection.
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