December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
Complement Activation via the Alternative Pathway Causes a Rapid-Onset Anterior Uveitis
Author Affiliations & Notes
  • JC Cruz
    Ophthalmology and Visual Sciences Kentucky Lions Eye Center University of Louisville Louisville KY
  • JH Sohn
    Louisville KY
  • HJ Suk
    Louisville KY
  • PS Bora
    Louisville KY
  • HJ Kaplan
    Louisville KY
  • NS Bora
    Louisville KY
  • Footnotes
    Commercial Relationships   J.C. Cruz, None; J.H. Sohn , None; H.J. Suk , None; P.S. Bora , None; H.J. Kaplan , None; N.S. Bora , None. Grant Identification: NIH EY13335, EY07930, EY13094, Research to Prevent Blindness, Inc, NYC, NY and Commonwealth of Kentu
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 2280. doi:
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      JC Cruz, JH Sohn, HJ Suk, PS Bora, HJ Kaplan, NS Bora; Complement Activation via the Alternative Pathway Causes a Rapid-Onset Anterior Uveitis . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2280.

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      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Abstract: : Purpose: To explore the role of the alternative pathway of complement in the development of complement-mediated uveitis. Methods: Lewis rats were divided into six groups (n=12/group). Rats in groups 1 and 2 were injected intracamerally with 5 and 50 µg zymosan suspension (5µl), respectively. Control rats (group 3) were injected with 5 µl of sterile PBS. Animals in groups 4 and 5 received purified cobra venom factor (CVF) i.p. 24 hours before receiving 5 and 50 µg zymosan, respectively. Polystyrene latex beads were used as control particles and group 6 (n=12) was injected intracamerally with 5 µl of this suspension. The development and severity of uveitis was monitored by clinical and histopathologic examination. The experiment was repeated twice. Results: Injection of zymosan (5 and 50 µg) into the anterior chamber of the eye induced severe anterior uveitis in all Lewis rats. With 5 µg of zymosan, maximum inflammation was observed between 24-48 hours and the disease resolved by day 9. However, with 50 µg of zymosan, severe inflammation persisted until day 10 with complete resolution by day 19. Histologically within 3 to 4 hours after injection of zymosan (5 or 50 µg), polymorphonuclear cells (PMNs) infiltrated the iris and ciliary body, with prominent swelling of the iris and ciliary body over the next 2 days. PMNs appeared in the anterior chamber, with spillover into the anterior vitreous; the cornea, retina and choroid was not inflamed. Pretreatment of Lewis rats with CVF completely suppressed the anterior uveitis inuced by zymosan. Intracameral injection of latex beads produced only a mild transient inflammatory reaction. Anterior uveitis did not develop in any of the animals injected with PBS. Conclusion: Our results demonstrate that the activation of the complement system via the alternative pathway can induce a severe complement-mediated anterior uveitis. The rapid onset of intraocular inflammation with a predominance of PMNs mimics certain subsets of clinical anterior uveitis which are distinct from experimental autoimmune uveits induced by uveal or retinal protein.

Keywords: 612 uveitis-clinical/animal model • 437 inflammation • 324 aqueous 

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