Purchase this article with an account.
KA Rezai, L Farrokh-Siar, EM Gasyna, SC Patel, G van Seventer, JT Ernest; The effect of IFN-alpha, IFN-gamma, and TGF-beta on retinal pigment epithelium induced apoptosis in Jurkat T-cells . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2287.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Purpose:To examine the up-regulatory effect of different cytokines on retinal pigment epithelium induced apoptosis in human Jurkat T-cells (Jkt). Methods:Pure cultures of human fetal retinal pigment epithelial cells (HFRPE) were isolated and cultured. HFRPE cells were incubated with IFN-a, IFN-g, TGF-b, or IFN-g and TGF-b simultaneously for 72 hours. Then the activated HFRPE cells were co-cultured with Jkt cells for 48 hours. Apoptosis of Jkt cells was determined with Annexin V staining and flowcytometry. Results:Both IFN-a and IFN-g activated HFRPE cells induced an increased amount of apoptosis in Jkt cells (20.51+2.4 % and 34.47+2.01 %) respectively. TGF-b activated HFRPE cells, however, did not induce a significant amount of apoptosis in Jkt cells (14.76+1.33 %) in comparison to non-activated HFRPE cells (15.68+2.31). The apopstosis rate in Jkt cells after incubation with both IFN-g and TGF-b activated HFRPE cells was similar to only IFN-g activated HFRPE cells (37.09+3.5 %). Conclusion:These results indicate that type I and type II IFNs can similarly up-regulated the HFRPE induced apoptosis in Jkt T-cells. TGF-b, however, could not up-regulate the HFRPE induced apoptosis in Jkt cells. These findings may help us to better understand the modulatory effect of pro- and anti-inflammatory cytokines on immune suppressive characteristics of HFRPE cells.
This PDF is available to Subscribers Only