December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Towards an Expression Profile of Native Human Retinal Pigment Epithelium and Identification of RPE-Specific Genes
S Zareparsi; M Buraczynska; AJ Mears; R Farjo; SP MacNee; B Hughes; A Swaroop
Author Affiliations & Notes
  • S Zareparsi
    Ophthalmology and Visual Sciences
    University of Michigan WK Kellogg Eye Center Ann Arbor MI
  • M Buraczynska
    Ophthalmology and Visual Sciences
    University of Michigan WK Kellogg Eye Center Ann Arbor MI
  • AJ Mears
    Ophthalmology and Visual Sciences
    University of Michigan WK Kellogg Eye Center Ann Arbor MI
  • R Farjo
    Ophthalmology and Visual Sciences
    University of Michigan WK Kellogg Eye Center Ann Arbor MI
  • SP MacNee
    Ophthalmology and Visual Sciences
    University of Michigan WK Kellogg Eye Center Ann Arbor MI
  • B Hughes
    Physiology
    University of Michigan WK Kellogg Eye Center Ann Arbor MI
  • A Swaroop
    Ophthalmology and Visual Sciences
    University of Michigan WK Kellogg Eye Center Ann Arbor MI
  • Footnotes
    Commercial Relationships   S. Zareparsi, None; M. Buraczynska, None; A.J. Mears, None; R. Farjo, None; S.P. MacNee, None; B. Hughes, None; A. Swaroop, None. Grant Identification: Support: NIH Grant EY07961, EY11115, EY07003 and M01-RR00042 and the MVRF, FFB and RPB
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 2434. doi:
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      S Zareparsi, M Buraczynska, AJ Mears, R Farjo, SP MacNee, B Hughes, A Swaroop; Towards an Expression Profile of Native Human Retinal Pigment Epithelium and Identification of RPE-Specific Genes . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2434.

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Abstract

Abstract: : Purpose: To generate a profile of genes expressed in the native human retinal pigment epithelium and to examine their tissue specific expression pattern to identify novel RPE-specific genes. Methods: An unamplified cDNA library was constructed using RNA isolated from native human RPE sheets. The sequence from the 5'end was obtained for randomly selected clones; of these, more than 1600 expressed sequence tags (ESTs) were analyzed for similarity to sequences and gene clusters in public databases. cDNA microarray slides containing RPE-expressed genes from this library will be generated and hybridized against RNA from RPE, retina and kidney. Results: EST analysis revealed several known RPE-expressed genes and more than 500 genes that have been characterized previously but not known to be expressed in the RPE. Over 200 novel ESTs and putative proteins have been identified. An additional 344 sequences only matched human genomic sequence. Comparative analysis of hybridizations with RNA from RPE, retina, and kidney will be performed in order to identify genes that are specifically expressed in the RPE. Conclusion: The analysis of ESTs generated from the native human RPE library has revealed a set of novel ESTs, putative proteins and genomic hits, which may represent as yet unidentified RPE-specific genes. Many of these map to the chromosomal regions of previously localized disease loci and may serve as candidate genes. Identification of novel RPE-specific genes will lead to a better understanding of the role that RPE plays in normal eyes and in disease state. In particular, RPE-specific genes will be studied as candidates for susceptibility to age-related macular degeneration.

Keywords: 567 retinal pigment epithelium • 417 gene/expression • 308 age-related macular degeneration 
© 2002, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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