Abstract
Abstract: :
Purpose: We studied the biochemical changes in high myopic vitreous to understand the vitreous degeneration and the reason why high myopic eyes are vulnerable to retinal detachment. Methods: The quantitative measure of protein, sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS/PAGE), Western Blot, uronic acid carbozole reaction were used to analyze soluble protein, hyaluronic acid and transferrin in the vitreous of 21 vitrectomy eyes of patients over 40 years old. Results: There is a positive correlation between protein and refraction (r=0.3308,p=0.006). The concentration of HA is lower (p=0.022) while that of transferrin is higher in high myopia (p≷0.05). Conclusion: Our study indicates the breakdown of blood-vitreous barrier in high myopic eyes.
Keywords: 481 myopia • 629 vitreous • 563 retinal detachment