December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
Author Affiliations & Notes
  • K Yamane
    Ophthalmology Hiroshima Univ Sch Med Hiroshima Japan
  • A Minamoto
    Ophthalmology Hiroshima Univ Sch Med Hiroshima Japan
  • HK Mishima
    Ophthalmology Hiroshima Univ Sch Med Hiroshima Japan
  • H Yamashita
    Ophthalmology Yamagata Univ Sch Med Yamagata Japan
  • H Tamamura
    Ophthalmology Yamagata Univ Sch Med Yamagata Japan
  • K Yoshizato
    Faculty of Science Hiroshima Univ Hiroshima Japan
  • Footnotes
    Commercial Relationships   K. Yamane, None; A. Minamoto, None; H.K. Mishima, None; H. Yamashita, None; H. Tamamura, None; K. Yoshizato, None. Grant Identification: Ministry of Health, Labor and Welfare, Japan
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 2459. doi:
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    • Get Citation

      K Yamane, A Minamoto, HK Mishima, H Yamashita, H Tamamura, K Yoshizato; PROTEOME ANALYSIS OF normal HUMAN VITREOUS fluid . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2459.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Purpose: To make a map to show protein expression in the normal vitreous, proteome survey was performed. This map can be used to detect the changes in protein expression levels in the pathological state. Methods: Vitreous samples and serum samples were obtained from the eyes with macular hole (23 cases) after securing the permission from the patients, who were informed of the nature of procedures. The expressed proteins in the vitreous sample and the serum sample from each patient were separated by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). The protein spots were visualized by silver staining and the protein expression patterns were analyzed by a computer associated image analysis system. Some protein spots were excised from the 2-D gels, digested in situ with trypsin, and analysis by mass spectrometry (Q-TOF mass spectrometer). Results: 200∼300 spots were detected on each 2-D gel. More than 20 protein/polypeptides were identified, including pigment epithelium-derived factor (PEDF), prostaglandin-D2 synthase, interphotoreceptor retinoid-binding protein precursor, which were unidentified in the corresponding serum samples. Conclusion: A map of the protein expression was made in the normal vitreous using proteome analysis. These expressed protein pattern in the vitreous was different from that in the corresponding serum, which suggest that the protein expression levels were determined by the ocular tissues and/or extraocular tissues.

Keywords: 526 protein purification and characterization • 629 vitreous • 461 macular holes 

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