December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Immunocytochemical Stains as Adjunct to Cytomorphology in Diagnosis of Intraocular Neoplasms Evaluated by Fine-needle Aspiration Biopsy
Author Affiliations & Notes
  • S Schneider
    University of Cincinnati Cincinnati OH
    Ophthalmology and Pathology & Laboratory Medicine
  • TB Lutz
    Ophthalmology
    University of Cincinnati Cincinnati OH
  • JJ Augsburger
    Ophthalmology
    University of Cincinnati Cincinnati OH
  • ZM Correa
    Ophthalmology ISCMPA-Santa Casa Porto Alegre Brazil
  • TM Robinson-Smith
    Pathology & Laboratory Medicine
    University of Cincinnati Cincinnati OH
  • RS Yassin
    Pathology & Laboratory Medicine
    University of Cincinnati Cincinnati OH
  • Footnotes
    Commercial Relationships   S. Schneider, None; T.B. Lutz, None; J.J. Augsburger, None; Z.M. Correa, None; T.M. Robinson-Smith, None; R.S. Yassin, None.
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 2578. doi:
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    • Get Citation

      S Schneider, TB Lutz, JJ Augsburger, ZM Correa, TM Robinson-Smith, RS Yassin; Immunocytochemical Stains as Adjunct to Cytomorphology in Diagnosis of Intraocular Neoplasms Evaluated by Fine-needle Aspiration Biopsy . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2578.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: To evaluate the efficacy of fine-needle aspiration biopsy (FNAB) to establish the diagnosis of metastatic intraocular tumor in cases of no known primary or prior insufficient aspirate from a remote biopsy site. Methods: Retrospective descriptive analysis of all intraocular FNAB specimens (n=11) obtained and evaluated at one center during the interval March 1999 through September 2001 in which the FNAB indication was amelanotic melanoma versus metastatic carcinoma. Results: Papanicolaou staining was performed on alcohol-fixed smears of the aspirates prepared in the operating room. Immunocytochemical staining was performed as indicated on the basis of review of the cytomorphic features. A positive diagnosis of malignancy was obtained in 10 cases; 7 were melanomas, 3 were metastases, 1 was insufficient for diagnosis. A primary site was identified in all cases diagnosed as metastasis with the aid of immunohistochemistry. Conclusion: FNAB reduces the uncertainty associated with problematic intraocular tumors. Immunocytochemical stains can be particularly helpful in interpretation of the pathologic nature of amelanotic neoplasms. Stains for melanocytic-derived tumors, epithelial tumors, neuroendocrine tumors, and others can be used to support or refute a diagnosis in atypical cases. Intraocular FNAB can be performed to establish a diagnosis of metastasis when biopsy of another bodily site is insufficient, relatively inaccessible, or has been nondiagnostic.

Keywords: 610 tumors • 508 pathology techniques • 496 oncology 
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