December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
Transscleral Permeability of Calcitriol, a new potential treatment for Retinoblastoma
Author Affiliations & Notes
  • C Hejny
    Ophthalmology Emory University Atlanta GA
  • LP J Cruysberg
    Ophthalmology Emory University Atlanta GA
  • DM Albert
    Ophthalmology University of Wisconsin-Madison Madison WI
  • DH Geroski
    Ophthalmology Emory University Atlanta GA
  • HF Edelhauser
    Ophthalmology Emory University Atlanta GA
  • Footnotes
    Commercial Relationships   C. Hejny, None; L.P.J. Cruysberg, None; D.M. Albert, None; D.H. Geroski, None; H.F. Edelhauser, None. Grant Identification: Support NEI grant P30-EY06360, T32-EY07096, R01-EY01917
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 2599. doi:
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      C Hejny, LP J Cruysberg, DM Albert, DH Geroski, HF Edelhauser; Transscleral Permeability of Calcitriol, a new potential treatment for Retinoblastoma . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2599.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract: : Background:Systemic administration of calcitriol has been demonstrated to cause a greater than 50% inhibition of retinoblastoma as compared to the controls in a mouse model. Use of this treatment in children has not been undertaken because of the associated toxicity related to hypercalcemia. Transscleral delivery of these agents has the potential to inhibit tumor growth without systemic toxicity. Purpose:To determine the in vitro scleral permeability of calcitriol. Methods:Scleral sections excised from moist-chamber stored human globes were mounted in a perfusion chamber. Transscleral pressure was maintained at 15 mm Hg and temperature was kept at 37 degrees Celsius. A small depot of drug (5.68 x 10-2 nanomoles/ml 3H-Calcitriol in BSS) was placed on the episcleral surface. BSS was perfused to the choroidal side for 24 hours. Fractions of choroidal perfusate were collected and radioactivity was measured with a scintillation counter. From this data, scleral permeability Ktrans (cm/sec) was calculated. Results:Ktrans for 3H-Calcitriol was 8.89 +/- 3.34 x 10-8 (mean +/- SE, n=8). Semi steady state was reached at 2 hours with a peak value of 3.88 x 10-6 nanomoles/ml reached at 3 hours. Conclusion:This study shows that delivery of 3H-Calcitriol in a physiologic solution across the human sclera can be achieved. Transscleral drug delivery of this drug for the treatment of retinoblastoma could possibly deliver higher intraocular concentrations than systemically and minimize side effects.

Keywords: 569 retinoblastoma • 514 pharmacology • 574 sclera 

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