December 2002
Volume 43, Issue 13
ARVO Annual Meeting Abstract  |   December 2002
ABCA1 Expression in Human RPE Cells and Age-Related Macular Degeneration
Author Affiliations & Notes
  • BD Sippy
    Emory Eye Center Atlanta GA
  • RK Shuler
    Emory Eye Center Atlanta GA
  • SJ Lee
    Emory Eye Center Atlanta GA
  • T Couse
    Winship Cancer Institute Atlanta GA
  • JN Wilcox
    Winship Cancer Institute Atlanta GA
  • JH Boatright
    Emory Eye Center Atlanta GA
  • JM Nickerson
    Emory Eye Center Atlanta GA
  • HE Grossniklaus
    Emory Eye Center Atlanta GA
  • Footnotes
    Commercial Relationships   B.D. Sippy, None; R.K. Shuler, None; S.J. Lee, None; T. Couse, None; J.N. Wilcox, None; J.H. Boatright, None; J.M. Nickerson, None; H.E. Grossniklaus, None. Grant Identification: Support: NIH Core Grant (P30 EY06360), RPB and the RPB-AOS-Knapp Ophthalmic Pathology Fellowship
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 2612. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      BD Sippy, RK Shuler, SJ Lee, T Couse, JN Wilcox, JH Boatright, JM Nickerson, HE Grossniklaus; ABCA1 Expression in Human RPE Cells and Age-Related Macular Degeneration . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2612.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Abstract: : Purpose: To demonstrate expression of ABCA1 in human RPE cells and to evaluate expression in age-related macular degeneration (ARMD). Methods: Primary human RPE cultures were obtained from donor tissue. Post-mortem eyes with and without histological evidence of ARMD were fixed, embedded in paraffin and sectioned. Real-time reverse transcriptase polymerase chain reaction (RT-PCR) assessed the presence of ABCA1 mRNA in total RNA isolates from RPE cells, and agarose gel electrophoresis confirmed RT-PCR product size. Tissue sections and cell culture slides were incubated with a radio-labeled probe specific for human ABCA1 mRNA using standard in situ techniques. Results: ABCA1 expression in human RPE cells was demonstrated by both RT-PCR and in situ hybridization. ABCA1 mRNA was evident in human retina, including the RPE monolayer, and was present in cells associated with ARMD lesions. Conclusion: We hypothesize that aberrant lipid metabolism in RPE cells may contribute to the pathogenesis of ARMD. Expression of ABCA1, an important gene in lipid metabolism, was evident in cultured human RPE cells and present in human retinal tissue with ARMD.

Keywords: 507 pathology: human • 417 gene/expression • 308 age-related macular degeneration 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.