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WA Beltran, JW Kijas, Q Zhang, GD Aguirre; Cloning and Expression of Ciliary Neurotrophic Factor Receptor (CNTFR) in the Canine Retina . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2679.
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© ARVO (1962-2015); The Authors (2016-present)
CNTF has a photoreceptor rescue effect in rodent and canine models of retinal degeneration. Its neuroprotective effect is thought to be mediated by the binding to its specific receptor, CNTFRα. Purpose: To clone the canine CNTFRα cDNA and examine its distribution in the canine adult retina. Method: RT-PCR was done using canine brain tissue as a source of mRNA and consensus primers designed from the human and mouse sequences. Immunohistochemistry was done on retinal cryosections using a commercial polyclonal antibody directed against human CNTFRα. Results: The canine CNTFRα cDNA comprises 1119 nucleotides (exons 3-9) and shares a high homology with both human (93%) and mouse (89%) sequences. At the amino acid level, the sequence is also highly conserved across the whole protein (98% homology with human, 95% with mouse) particularly in the cytokine receptor-like domain (98% homology with human and mouse). Immunohistochemical studies show an intense labeling at the level of the photoreceptors inner/outer segments, and at the outer plexiform, inner plexiform, ganglion cell, and nerve fiber layers. Conclusions: CNTFRα appears to be localized on photoreceptors suggesting that CNTF's rescue effect may occur through a direct binding mechanism on photoreceptors. In situ hybridization studies will determine whether the cells that transcribe CNTFRα are also the cells that express the receptor. CR: None Support: EY13132, Foundation Fighting Blindness, Morris Animal Foundation/The Seeing Eye, Inc., Van Sloun Fund for Canine Genetic Research
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