December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
A Gradient in Fgf-2 Expression During Formation of the Foveal Depression Correlates With Cone Morphology
EE G Cornish; AM Hales; AE Hendrickson; JM Provis
Author Affiliations & Notes
  • EE G Cornish
    Save Sight Institute and Anatomy & Histology The University of Sydney Sydney Australia
  • AM Hales
    Save Sight Institute and Anatomy & Histology The University of Sydney Sydney Australia
  • AE Hendrickson
    Biological Structure University of Washington Seattle WA
  • JM Provis
    Save Sight Institute and Anatomy & Histology The University of Sydney Sydney Australia
  • Footnotes
    Commercial Relationships   E.E.G. Cornish, None; A.M. Hales, None; A.E. Hendrickson, None; J.M. Provis, None. Grant Identification: NHMRC #153825; ORIA; Claffy Foundation (EC); Kayser Award
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 2684. doi:
  • Views
  • Share
  • Tools
    • Alerts
      User Alerts
      You are adding an alert for:
      A Gradient in Fgf-2 Expression During Formation of the Foveal Depression Correlates With Cone Morphology
      You will receive an email whenever this article is corrected, updated, or cited in the literature. You can manage this and all other alerts in My Account
      The alert will be sent to:
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation
      Citation

      EE G Cornish, AM Hales, AE Hendrickson, JM Provis; A Gradient in Fgf-2 Expression During Formation of the Foveal Depression Correlates With Cone Morphology . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2684.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

      ×
    • Get Permissions
  • Supplements
Abstract

Abstract: : Purpose:FGF-2 and the receptors flg and bek are expressed by cone photoreceptors during development. Foveal cones go through a period of delayed maturation during formation of the foveal depression1 but are transformed into a narrow and elongated phoenotype, postnatally. In this study we examined expression of FGF-2, flg and bek in relation to the morphological differentiation of foveal cones. Methods:Macaque retinae aged between foetal day (Fd) 65 and 4 months postnatal, were fixed in Methyl Carnoy's, embedded in paraffin and sectioned parallel to the horizontal meridian. DIG-labelled riboprobes were prepared from rat FGF-2, FGFR1 (flg) and R2 (bek) cDNAs (≷88% homology) for in situ hybridisation. Levels of mRNA expression were compared within sections using NIH Image and the Kruskal-Wallace and Dunnett-like statistical tests. Antibodies to the splice variants of R1 (flg) and R2 (bek) were used to demonstrate the post-translation distribution of R1 and R2. Results:In the post-mitotic monkey retina FGF-2 mRNA is virtually restricted to ganglion cells and cone photoreceptors. Immunoreactivity to flg (flg-IR) and bek-IR is present on cones, each with a distinctive distribution. Early in the formation of the foveal depression (Fd 115) there is a significant downregulation of FGF-2 mRNA within the foveal cone mosaic; morphologically, foveal cones are shorter and broader than in the parafovea at this age. In the same period flg-IR is intense on the foveal rim at the cone/RPE interface and at the level of the synapses, but is almost completely absent within the foveal cone mosaic. Bek-IR is maintained during early formation of the fovea and there is no evidence of bek mRNA downregulation. Postnatally FGF2 and both receptor mRNAs are expressed uniformly throughout the foveal cone mosaic. Conclusion:FGF receptor activation has a role in the morphological differentation of foveal cones. 1. Abramov et al.Science (1982)217:265-7.

Keywords: 517 photoreceptors • 423 growth factors/growth factor receptors • 564 retinal development 
© 2002, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×
This site uses cookies. By continuing to use our website, you are agreeing to our privacy policy.Accept