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PF Gardino, KC Calaza, PH O C Barros; GABAABeta 2-3 Immunoreactive Cells in the Developing Chick Retina . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2700.
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© ARVO (1962-2015); The Authors (2016-present)
Purpose: It has been shown that GABA is an important factor for maturation of the CNS. GABA functions during development of the CNS are mainly mediated by GABAA receptor (GABAAR). It is clearly important to establish the developmental features of the catalytics subunits (ß) of GABAAR in order to fully comprehend GABA role during development. Therefore, the aim of this work is to determine the neurogenesis and ontogenesis of cells expressing ß2-3 subunits of GABAAR in the chick retina. Methods: For neurogenesis, an association of autoradiography and immunohistochemistry techniques was applied. [H3]-thymidine was injected into eggs (from 2 to 11 days) and the embryos were sacrificed at embryonic day 18 (E18). Then, GABAAß2-3 immunohistochemistry was processed followed by autoradiography. For ontogenesis only immunohistochemistry was employed. Results: GABAAß2-3 amacrine cells proliferate between E6/7 and E9 suggesting that a trigger signal is required around E6/7. The ontogenesis revealed that at E8, GABAAß2-3 immunoreactivity (GABAAß2-3-IR) was restricted to the inner plexiform layer. At E14 the first cell bodies immunoreactive to GABAAß2-3 were seen. Thereafter, the number of cell bodies and the intensity of GABAAß2-3 -IR increased to reach the adult pattern. Conclusion: The detection of GABAAß2-3 -IR in cell bodies five days after the end of neurogenesis supports the idea that a specific signal is necessary to induce GABAAß2-3 subunits expression in amacrine cells.
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