December 2002
Volume 43, Issue 13
Free
ARVO Annual Meeting Abstract  |   December 2002
Rat Strain Difference in Ischemic Regulation of VEGF and PEDF and in Susceptibilities to Retinal Neovascularization
Author Affiliations & Notes
  • G Gao
    Ophthalmology Storm Eye Institute Charleston SC
  • Y Li
    Ophthalmology Storm Eye Institute Charleston SC
  • J Fant
    Ophthalmology Storm Eye Institute Charleston SC
  • CE Crosson
    Ophthalmology Storm Eye Institute Charleston SC
  • SP Becerra
    National Eye Institute Laboratory of Retinal cell and Molecular Biology Bethesda MD
  • JX Ma
    Ophthalmology Storm Eye Institute Charleston SC
  • Footnotes
    Commercial Relationships   G. Gao, None; Y. Li, None; J. Fant, None; C.E. Crosson, None; S.P. Becerra, None; J.X. Ma, None. Grant Identification: NIH EY12600, NIH EY09741, JDF, ADA and RPB
Investigative Ophthalmology & Visual Science December 2002, Vol.43, 2754. doi:
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    • Get Citation

      G Gao, Y Li, J Fant, CE Crosson, SP Becerra, JX Ma; Rat Strain Difference in Ischemic Regulation of VEGF and PEDF and in Susceptibilities to Retinal Neovascularization . Invest. Ophthalmol. Vis. Sci. 2002;43(13):2754.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Abstract: : Purpose: Sprague Dawley (SD) rats are more resistant to ischemia-induced retinal neovascularization (IRN) compared to Brown Norway (BN) rats. The purpose of this study is to understand the molecular basis responsible for the rat strain difference. Methods: IRN was produced by exposure of newborn BN or SD rats to 75% oxygen for 7 days followed by room air. Retinal neovascularization was evaluated by means of angiography and vascular cell counts in the neural retina cross-sections. Changes in mRNA and protein levels of vascular endothelial growth factor (VEGF) and pigment epithelium-derived factor (PEDF) were measured in the retina by Northern and Western blot analyses at selected time following return of rats to roomair. Results: Exposure to constant hyperoxia followed by normoxia induced significant retinal neovascularization in BN rats, but not in SD rats. These results confirmed a strain difference in susceptibility to IRN in rat retina. BN rats exposed to hyperoxia showed a 50% reduction in retinal PEDF, and a 5-fold increase of VEGF at the protein level, resulting in an increased VEGF/PEDF ratio. The similar changes were also observed at mRNA level in BN rats with IRN. However, SD rats exposed to hyperoxia showed less and shorter changes in PEDF and VEGF levels than that in BN rats. In age-matched normal BN and SD rats, there was no detectable difference in the basal VEGF/PEDF ratio between the strains. Conclusions: These studies provide initial evidence that different regulations of angiogenic inhibitors and stimulators under ischemia are responsible for the differences in susceptibility to IRN in SD and BN rats. The pigmented BN rat is more suitable for the ischemia-induced retinopathy model.

Keywords: 566 retinal neovascularization • 316 animal model • 423 growth factors/growth factor receptors 
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